Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TXNIP Polyclonal Antibody, Unconjugated(bs-3897R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-3897R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3897R |
| 英文名称 | TXNIP Rabbit pAb |
| 中文名称 | 硫氧还蛋白结合蛋白2抗体 |
| 英文别名 | ; THIF; thioredoxin binding protein 2; thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; upregulated by 1, 25-dihydroxyvitamin D-3; VDUP1; Vitamin D3 up-regulated protein 1; TXNIP_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TXNIP |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Cancer > Oncoproteins/suppressors > Tumor suppressors Cell Biology > Other Antibodies > Oxidative Stress Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Co-activators/co-repressors Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4. |
| 亚细胞定位 | Cytoplasm |
| 翻译后修饰 | Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation. |
| 相似性 | Belongs to the arrestin family. |
| 功能 | May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | TXNIP may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. It interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. It functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. It is required for the maturation of natural killer cells (from SwissProt). |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 5 }}] {Shi Ao. et al. NLRP3-inflammasome Related Genes as Emerging Biomarkers and Therapeutic Targets in Psoriasis. INFLAMMATION. 2025 Mar;:1-13} {IHC,IF} {Human,Mouse}
[IF={{ 3.2 }}] {Jianwen Fei. et al. TXNIP activates NLRP3/IL-1β and participate in inflammatory response and oxidative stress to promote deep venous thrombosis. EXP BIOL MED. ;():} {WB} {Rat}
[IF={{ 2.9 }}] {Li, Xiao-Feng, et al. "MicroRNA-20a negatively regulates expression of NLRP3-inflammasome by targeting TXNIP in adjuvant-induced arthritis fibroblast-like synoviocytes." Joint Bone Spine (2016).} {WB} {="Human"}
[IF={{ 2.86 }}] {Liu, Zhenning, et al. "Silymarin attenuated paraquat-induced cytotoxicity in macrophage by regulating Trx/TXNIP complex, inhibiting NLRP3 inflammasome activation and apoptosis." Toxicology in Vitro (2017).} {WB,ICC} {="Mouse"}
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TXNIP Rabbit pAb(bs-3897R)-50ul/100ul/200ul
￥1180 - 2800
