Sample:
BRL-3A(Rat) Cell Lysate at 30 ug
Primary: Anti-HNF4A (bs-3828R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
- ￥1180 - 2800
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- bs-3828R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3828R |
| 英文名称 | HNF4A Rabbit pAb |
| 中文名称 | 肝细胞核因子4α抗体 |
| 英文别名 | FLJ39654; HNF-4-alpha; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4. |
| 产品应用 | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human HNF4 alpha |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Blood > Coagulation > Extrinsic Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cholesterol Metabolism Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cytochromes Developmental Biology > Organogenesis > Gut development > Liver development Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Cholesterol Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipases Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Mitochondrial Metabolism > Cytochromes Metabolism > Types of disease > Cancer Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Homodimerization is required for HNF4-alpha to bind to its recognition site. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. |
| 相似性 | Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain. |
| 功能 | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HNF4A Polyclonal Antibody, Unconjugated(bs-3828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HNF4A Polyclonal Antibody, Unconjugated(bs-3828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HNF4A) Polyclonal Antibody, Unconjugated (bs-3828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: Hcclm3 Cell (Human) Lysate at 40 ug
Primary: Anti- HNF4A (bs-3828R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Primary: Anti- HNF4A (bs-3828R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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文献和实验该产品被引用文献
[IF={{ 9.6 }}] {Yang Yu-Hang. et al. HNF4A mitigates sepsis-associated lung injury by upregulating NCOR2/GR/STAB1 axis and promoting macrophage polarization towards M2 phenotype. CELL DEATH DIS. 2025 Feb;16(1):1-14} {WB,IF,ChIP} {Mouse}
[IF={{ 7.8 }}] {Kenji Uno. et al.Rspo3-mediated metabolic liver zonation regulates systemic glucose metabolism and body mass in mice.PLOS BIOLOGY.2025 Jan 24;23(1):e3002955.} {Fluorescent immunostaining} {Murine}
[IF={{ 4.099 }}] {Lv, Xiaoqin. et al. GATA binding protein 4 promotes the expression and transcription of hepatitis B virus by facilitating hepatocyte nuclear factor 4 alpha in vitro. Virol J. 2021 Dec;18(1):1-12} {WB} {human}
[IF={{ 3.776 }}] {Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs. HELIYON. 2022 Dec;8:e12385} {IF,WB} {Human}
[IF={{ 2.523 }}] {Zhang C et al. Caveolin-1 promotes Rfng expression via Erk-Jnk-p38 signaling pathway in mouse hepatocarcinoma cells. J Physiol Biochem. 2019 Sep 16.} {WB} {Mouse}
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HNF4A Rabbit pAb(bs-3828R)-50ul/100ul/200ul
￥1180 - 2800
