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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0516R |
| 英文名称 | RARB Rabbit pAb |
| 中文名称 | 维甲酸受体β抗体 |
| 英文别名 | Retinoic acid R-beta; HAP; HBV activated protein; HBV-activated protein; Hepatitis B virus activated protein; NR1B2; Nuclear receptor subfamily 1 group B member 2; RAR B; RAR beta; RAR epsilon; Retinoic acid receptor beta 2; Retinoic acid receptor beta 4; Retinoic acid receptor beta 5; Retinoic acid receptor beta; Retinoic acid receptor beta polypeptide; RRB 2; RARB_HUMAN; RRB2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RARB |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction > Nuclear signaling > Nuclear hormone receptors > Retanoic and retanoid Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Retinoic & Retinoid Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Retinoic & Retinoid |
| 亚基 | Homodimer (By similarity). Heterodimer; with a RXR molecule. Binds DNA preferentially as a RAR/RXR heterodimer (By similarity). Interacts weakly with NCOR2. |
| 亚细胞定位 | Isoform Beta-1: Nucleus. Isoform Beta-2: Nucleus. Isoform Beta-4: Cytoplasm. |
| 相似性 | Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. |
| 功能 | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Retinoids are metabolites of vitamin A (retinal) and are believed to represent important signaling molecules during vertebrate development and tissue differentiation. Two families of retinoid receptors have been identified. Retinoic acid receptors (RARs) include RAR alpha, RAR Beta and RAR gamma, each of which has a high affinity for all trans retinoic acids and belongs to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. The ligand binding domains of the RARs are highly conserved and RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. Members of the retinoid X receptor (RXR) family, RXR alpha, RXR Beta and RXR gamma, are activated by 9 cis retinoic acid, a stereo and photoisomer of all trans RA that is expressed in vivo in both liver and kidney and may represent a widely used hormone. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (RAR Beta) polyclonal Antibody, Unconjugated (bs-0516R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Sample:
_x000D_ Heart (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-RAR Beta (bs-0516R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kD
_x000D_ Observed band size: 50 kD
_x000D_ Heart (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-RAR Beta (bs-0516R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kD
_x000D_ Observed band size: 50 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-RAR Beta Polyclonal Antibody, Unconjugated(bs-0516) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-RAR Beta Polyclonal Antibody, Unconjugated(bs-0516) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Blank control: SHSY5Y.
_x000D_ Primary Antibody (green line): Rabbit Anti-RAR Beta antibody (bs-0516R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-RAR Beta antibody (bs-0516R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (bs-0516R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (bs-0516R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (bs-0516R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (bs-0516R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (bs-0516R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-RAR Beta (bs-0516R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kD
_x000D_ Observed band size: 50 kD
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-RAR Beta (bs-0516R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kD
_x000D_ Observed band size: 50 kD
Sample:
_x000D_ Lane 1: Mouse Eye tissue lysates
_x000D_ Lane 2: Mouse Cerebrum tissue lysates
_x000D_ Primary: Anti- RAR Beta (bs-0516R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti- Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kDa
_x000D_ Observed band size: 50 kDa
_x000D_
_x000D_ Lane 1: Mouse Eye tissue lysates
_x000D_ Lane 2: Mouse Cerebrum tissue lysates
_x000D_ Primary: Anti- RAR Beta (bs-0516R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti- Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 50 kDa
_x000D_ Observed band size: 50 kDa
_x000D_
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RARB | RAR-beta Rabbit pAb(bs-0516R)-50ul/100ul/200ul
￥1180 - 2800
