Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated(bs-1288R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-1288R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1288R |
| 英文名称 | GDF8 Rabbit pAb |
| 中文名称 | 生长分化因子8抗体 |
| 英文别名 | GDF 8; GDF-8; GDF8_HUMAN; Growth differentiation factor 8; Growth/Differentiation Factor 8; MSTN; myostatin; OTTHUMP00000163498. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GDF8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 12/43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homodimer; disulfide-linked. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Predominantly expressed in muscle. At hatching, expression is strongest in the skin epithelium, and is also found in the retina and brain. From day 28, expressed in skeletal muscle. In the adult, highest expression is seen in the gastrointestinal tract, brain, muscle, heart and testis. Also expressed in the adult pharynx, kidney, spleen, liver, gill, eyes, skin, swim bladder and ovary. |
| 相似性 | Belongs to the TGF-beta family. |
| 功能 | Acts specifically as a negative regulator of skeletal muscle growth. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Myostatin (GDF8)is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ Lane 1: Muscle (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Muscle (Rat) Lysate at 40 ug
_x000D_ Lane 3: Tongue (Rat) Lysate at 40 ug
_x000D_ Primary:
_x000D_ Anti-GDF8 (bs-1288R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 43 kD
_x000D_ Observed band size: 43 kD
_x000D_ Lane 1: Muscle (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Muscle (Rat) Lysate at 40 ug
_x000D_ Lane 3: Tongue (Rat) Lysate at 40 ug
_x000D_ Primary:
_x000D_ Anti-GDF8 (bs-1288R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 43 kD
_x000D_ Observed band size: 43 kD
Sample:
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 12/43 kD
_x000D_ Observed band size: 43 kD
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 12/43 kD
_x000D_ Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858 WB Mouse
[IF=4.366] Genxi Zhang. et al. MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation. Cells-Basel. 2021 Feb;10(2):423 WB Chicken
[IF=4.26] Qian et al. Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs. (2015) Sci.Re. 5:14435 WB Pig
[IF=3.5] Yiting Kang. et al. Weight-Bearing Ladder Climbing Exercise Improves Bone Loss and Bone Microstructural Damage While Promoting Bone Injury Healing in OVX Rats. BIOLOGY-BASEL. 2025 Dec;15(1):55 WB Rat
[IF=2.788] Jin, Zhengyun. et al. Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs. TRANSGENIC RES. 2022 May;:1-9 WB Pig
相关实验
【热门靶点解析】GDF-8的生物学功能、疾病关联及相关产品推荐
肌生长抑制素（Myostatin, MSTN），即生长分化因子8（GDF-8），属于TGF-β超家族，是骨骼肌发育的关键负调控因子。1997年，McPherron等人首次鉴定出GDF-8，发现其主要在胚胎发育早期的骨骼肌组织中特异性表达。研究证实，当GDF-8基因发生靶向突变时，小鼠骨骼肌生长显著增强，肌肉质量普遍增加，可达野生型小鼠的2至3倍。这一发现明确揭示了GDF-8在抑制肌肉生长中的核心作用，表明其是调控肌肉发育的重要分子开关。 GDF8表达分布 肌生长抑制素（MSTN）不仅在骨骼
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MSTN | GDF8 Rabbit pAb(bs-1288R)-50ul/100ul/200ul
￥1180 - 2800
