Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated(bs-1288R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-1288R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1288R |
| 英文名称 | GDF8 Rabbit pAb |
| 中文名称 | 生长分化因子8抗体 |
| 英文别名 | GDF 8; GDF-8; GDF8_HUMAN; Growth differentiation factor 8; Growth/Differentiation Factor 8; MSTN; myostatin; OTTHUMP00000163498. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GDF8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 12/43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homodimer; disulfide-linked. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Predominantly expressed in muscle. At hatching, expression is strongest in the skin epithelium, and is also found in the retina and brain. From day 28, expressed in skeletal muscle. In the adult, highest expression is seen in the gastrointestinal tract, brain, muscle, heart and testis. Also expressed in the adult pharynx, kidney, spleen, liver, gill, eyes, skin, swim bladder and ovary. |
| 相似性 | Belongs to the TGF-beta family. |
| 功能 | Acts specifically as a negative regulator of skeletal muscle growth. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Myostatin (GDF8)is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Muscle (Rat) Lysate at 40 ug
Lane 3: Tongue (Rat) Lysate at 40 ug
Primary:
Anti-GDF8 (bs-1288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Muscle (Rat) Lysate at 40 ug
Lane 3: Tongue (Rat) Lysate at 40 ug
Primary:
Anti-GDF8 (bs-1288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/43 kD
Observed band size: 43 kD
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 8.713 }}] {Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858} {WB} {Mouse}
[IF={{ 4.366 }}] {Genxi Zhang. et al. MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation. Cells-Basel. 2021 Feb;10(2):423} {WB} {Chicken}
[IF={{ 4.26 }}] {Qian et al. Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs. (2015) Sci.Re. 5:14435} {WB} {Pig}
[IF={{ 2.788 }}] {Jin, Zhengyun. et al. Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs. TRANSGENIC RES. 2022 May;:1-9} {WB} {Pig}
[IF={{ 2.59 }}] {Yamada A.K.. et al. Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway. Braz J Med Biol Res. 2022 Jan;55:} {WB} {Rat}
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GDF8 Rabbit pAb(bs-1288R)-50ul/100ul/200ul
￥1180 - 2800
