NFASC | Neurofascin Rabbit pAb(bs-0289R)-50ul/100ul/200ul产品图

NFASC | Neurofascin Rabbit pAb

(bs-0289R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-0289R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-0289R
    英文名称Neurofascin Rabbit pAb
    中文名称神经束蛋白抗体
    英文别名KIAA0756; Neurofascin; Neurofascin homolog; NF; Nfasc; Nfasc protein; NFASC_HUMAN; NRCAML; neurofascin 155 kDa isoform; Neurofascin155; Neurofascin-155; NF-155.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human, Chicken, Dog, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Neurofascin-155
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量132/150 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Cell Type Marker > Neuron marker > Axon marker

    Neuroscience > Cell Type Marker > Neuron marker > Growth Cone

    Neuroscience > Neurology process > Growth and Development > Axonal Guidance Proteins

    Neuroscience > Neurology process > Neurogenesis

    亚基Horseshoe-shaped homodimer.
    亚细胞定位Cell membrane; Single-pass type I membrane protein.
    相似性Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    功能Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Neurofascin is a cell adhesion molecule involved in mediating axon recognition but also signaling axonal contact. Immunoglobulin domain cell adhesion molecule (cam) subfamily; members are components of neural cell adhesion molecules (N-CAM L1), Fasciclin II and the insect immune protein Hemolin. The subfamily also includes receptor domains such as as the extracelluar ligand binding domain of Fibroblast Growth Factor Receptor 2. Members are phylogenetically diverse, occuring throughout metazoa, and are not components of the adaptive immune system molecules found in jawed vertebrates. A predominant feature of most Ig domains is a disulfide bridge connecting 2 beta-sheets with a Trp packing against the disulfide bond.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: rat spinal cord tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
    _x000D_ Incubation: Anti-Neurofascin Polyclonal Antibody, Unconjugated(bs-0289R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Tissue/cell: Rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
    _x000D_ Incubation: Anti-Neurofascin Polyclonal Antibody, Unconjugated(bs-0289R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Sample: Cerebellum (Mouse) Lysate at 30 ug
    _x000D_ Primary: Anti- Neurofascin Polyclonal (bs-0289R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 132/150 kD
    _x000D_ Observed band size: 136 kD
    _x000D_
    产品细节图片4
    Sample:
    _x000D_ Lane 1: Cerebrum (Mouse) Lysate at 40 ug
    _x000D_ Lane 2: Cerebellum (Mouse) Lysate at 40 ug
    _x000D_ Lane 3: Cerebrum (Rat) Lysate at 40 ug
    _x000D_ Lane 4: Cerebellum (Rat) Lysate at 40 ug
    _x000D_ Primary: Anti-Neurofascin (bs-0289R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 132 kD
    _x000D_ Observed band size: 132 kD
    产品细节图片5
    Sample:
    _x000D_ Cerebral cortex (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-Neurofascin (bs-0289R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 132/150 kD
    _x000D_ Observed band size: 150 kD

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