Blank control: A549(blue), the cells were fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
- ￥1180 - 2800
- Bioss
- bs-6982R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
PDGFRA Rabbit pAb, PE conjugated(bs-0231R-PE)-100ul
￥2980
Cytochrome C Mouse mAb, APC-Cy7 conjugated(bsm-33193M-APC-Cy7)-100ul
￥2980
LCN9 Rabbit pAb(bs-18197R)-50ul/100ul/200ul
￥1180Desmocollin 2 + 3 Rabbit pAb, FITC conjugated(bs-2983R-FITC)-100ul
￥2980
HBA1 Recombinant Rabbit mAb(bsm-60287R)-50ul/100ul/25ul
￥800
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6982R |
| 英文名称 | Neutrophil Elastase Rabbit pAb |
| 中文名称 | 中性粒细胞弹性蛋白酶ELANE抗体 |
| 英文别名 | Bone Marrow Serine Protease; ELA 2; ELA2; ELANE; Elastase 2; Elastase-2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE antibody Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Leukocyte Elastase Precursor; Medullasin; NE; Neutrophil elastase; Neutrophill elastase; PMN E; PMN Elastase; Polymorphonuclear elastase; SCN1; Serine protease; SERP1. |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Neutrophil Elastase/ELANE |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 26 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Extracellular matrix Immunology > Innate Immunity > Complement > Regulatory Immunology > Innate Immunity > Macrophage / Inflamm. Immunology > Innate Immunity > NK Cells Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Adhesion molecules ELISA kits Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Angiogenic factors ELISA kits Microbiology > Protein > Human Protein > Defensin Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Enzymes > Other Enzymes Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Elastin Signal Transduction > Signaling Pathway > Calcium Signaling |
| 亚基 | Interacts with NOTCH2NL. |
| 组织特异性 | Bone marrow cells. |
| 相似性 | Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. |
| 功能 | Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (bs-6982R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (bs-6982R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 9.8 }}] {Mor Zigdon. et al. Salmonella manipulates the host to drive pathogenicity via induction of interleukin 1β. PLOS BIOL. 2024 Jan;22(1):e3002486} {IHC} {Mouse}
[IF={{ 8.44 }}] {Li Xiangdong. et al. Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. J TRANSL MED. 2023 Dec;21(1):1-19} {WB} {Mouse}
[IF={{ 7.793 }}] {Wang H et al. The effect of exposure time and concentration of airborne PM2. 5 on lung injury in mice: A transcriptome analysis. Redox Biol. 2019 Jul 2;26:101264.} {IHC} {Mouse}
[IF={{ 7.793 }}] {Gao J et al. Metformin protects against PM2. 5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2019 Oct 19;28:101345.} {IHC} {Mouse}
[IF={{ 7.3 }}] {Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439} {IHC,WB} {Rat}
相关实验
ELISA 96T 1000 进口分装 F0201 中性粒细胞活化肽(NAP) ELISA 48T/96T 2000/3000 进口分装 F0202 噬中性及细胞活化肽-2(NAP-2) ELISA 48T/96T 2000/3000 进口分装 F0203 中心粒细胞弹性蛋白酶(Neutrophil elastase) ELISA 48T/96T 2000/3000 进口分装 F0204 ★ 神经生长因子(NGF) ELISA 48T
ELISA 96T 1000 进口分装 F0201 中性粒细胞活化肽(NAP) ELISA 48T/96T 2000/3000 进口分装 F0202 噬中性及细胞活化肽-2(NAP-2) ELISA 48T/96T 2000/3000 进口分装 F0203 中心粒细胞弹性蛋白酶(Neutrophil elastase) ELISA 48T/96T 2000/3000 进口分装 F0204 ★ 神经生长因子(NGF) ELISA 48T
技术资料暂无技术资料 索取技术资料
文献支持
Neutrophil Elastase Rabbit pAb(bs-6982R)-50ul/100ul/200ul
￥1180 - 2800
