Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-OATP-1/Slco1a1 Polyclonal Antibody, Unconjugated(bs-0607R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-0607R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0607R |
| 英文名称 | Slco1a1 Rabbit pAb |
| 中文名称 | 溶质载体有机阴离子转运蛋白家族成员1A1抗体 |
| 英文别名 | OATP-1; Organic anion-transporting polypeptide 1; SO1A1_RAT; Sodium-independent organic anion transporter 1; Solute carrier family 21 member 1; Solute carrier organic anion transporter family member 1A1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from rat Slco1a1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 74 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds to PDZK1. Interaction with PDZK1 is required for expression on hepatocyte surface. |
| 亚细胞定位 | Basolateral cell membrane |
| 组织特异性 | Highly expressed in liver and kidney, and at lower levels in brain, lung, skeletal muscle and proximal colon. |
| 翻译后修饰 | Glycosylated. |
| 相似性 | Belongs to the organo anion transporter (TC 2.A.60) family._x000D_
[SIMILARITY] Contains 1 Kazal-like domain. |
| 功能 | Mediates the Na(+)-independent transport of organic anions such as bromosulfobromophthalein (BSP) and conjugated (taurocholate) and unconjugated (cholate) bile acids. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | mediates the transport of sulfated, amidated bile acid, sulfolithocholyltaurine, into rat hepatocytes |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ Liver (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Slco1a1 (bs-0607R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 74 kD
_x000D_ Observed band size: 73 kD
_x000D_ Liver (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Slco1a1 (bs-0607R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 74 kD
_x000D_ Observed band size: 73 kD
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文献和实验该产品被引用文献
[IF=6.876] Song Guochao. et al. Potential therapeutic action of tauroursodeoxycholic acid against cholestatic liver injury via hepatic Fxr/Nrf2 and CHOP-DR5-caspase-8 pathway. CLIN SCI. 2023 Apr;137(7):561-577 WB Mouse
[IF=5.23] Enoki, Yuki, et al. "Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1." Scientific Reports 6 (2016): 32084. WB ="Mouse"
[IF=3.3] Xu C, Zhu L, Chan T, Lu X, Shen W, et al. (2015) The Altered Renal and Hepatic Expression of Solute Carrier Transporters (SLCs) in Type 1 Diabetic Mice. PLoS ONE 10(3): e0120760. WB ="Mouse"
[IF=2.76] Zhu, Liran, et al. "Saponins extracted from Dioscorea collettii rhizomes regulate the expression of urate transporters in chronic hyperuricemia rats." Biomedicine & Pharmacotherapy 93 (2017): 88-94. IHC ="Rat"
[IF=1.585] Pang et al. Gypenosides Inhibits Xanthine Oxidoreductase and Ameliorates Urate Excretion in Hyperuricemic Rats Induced by High Cholesterol and High Fat Food (Lipid Emulsion). (2017) Med.Sci.Monit. 23:1129-1140 IHC Rat
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Slco1a1 | Oatp1 Rabbit pAb(bs-0607R)-50ul/100ul/200ul
￥1180 - 2800
