- ￥1180 - 2800
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- bs-4507R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4507R |
| 英文名称 | PRRSV M protein Rabbit pAb |
| 中文名称 | 猪蓝耳病病毒M蛋白抗体 |
| 英文别名 | M protein; envelope protein; M matrix protein; ORF6; non-glycosylated membrane protein; PRRSV-M; PRRSV-M protein. |
| 中文别名 | 猪繁殖与呼吸综合征病毒M蛋白; |
| 产品应用 | ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Pig, PRRSV) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from PRRSV M protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 20 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PRRSV is a small, enveloped RNA virus. It contains a single-stranded, positive-sense, RNA genome with a size of approximately 15 kilobases. The genome contains nine open reading frames. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. Subclinical infections are common, with clinical signs occurring sporadically in a herd. Clinical signs include reproductive failure in sows such as abortions and giving birth to stillborn or mummified fetuses, and cyanosis of the ear and vulva. In neonatal pigs, the disease causes respiratory distress, with increased susceptibility to respiratory infections such as Glasser's disease. |
| 应用 | 推荐稀释比例 |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 5.64 }}] {Zhao J. et al. The Construction and Immunogenicity Analyses of Recombinant Pseudorabies Virus With NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus-Like Particles Co-expression.. Front Microbiol. 2022 Mar;13:846079-846079} {IF,WB} {Hamster}
[IF={{ 2.57 }}] {Zhou, Mengjiao, et al. "miRNA29 Promotes Viral Replication During Early Stage of PRRSV Infection In Vitro." DNA and Cell Biology (2016).} {WB} {="Pig"}
[IF={{ 2.11 }}] {Binjawadagi, Basavaraj, et al. "Development of a porcine reproductive and respiratory syndrome virus-like-particle-based vaccine and evaluation of its immunogenicity in pigs." Archives of Virology: 1-11.} {Other} {=""}
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
., Finstad, J., Williams, Jr., R. C. 1970. Phylogenetic insight into evolution of mammalian Fc fragment of g G globulin using staphylococcal protein A. J. Immunol. 104:140-147. Kronvall, G. 1973. A surface component in group A, C, and G streptococci
CHART 1. take ~350 gram rabbit skeletal muscle 2. extract at +4 oC with 0.1 M KCl and filter 3. (take residue) extract at +4 oC with 50 mM NaHCO3 and filter 4. (take residue) extract at +4 oC with 1 mM EDTA, pH 7.0 and filter
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
(sulfosuccinimidyl) suberate (BS3; see recipe ) Dimethylsulfoxide (DMSO; H 2 O‐free, i.e., fresh) Quenching buffer: 1 M Tris·Cl, pH
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