Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Polyclonal Antibody, Unconjugated (bs-4690R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated(bs-4690R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
已发表文献 [IF=23.394]Fletcher, Russell B., et al. "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." Cell Stem Cell (2017).FCM, Mouse [IF=7.13]Gleeson, Birgitta M., et al. "Bone marrow‐derived mesenchymal stem cells have innate procoagulant activity and cause microvascular obstruction following intracoronary delivery: Amelioration by anti‐thrombin therapy." STEM CELLS (2015)., Pig [IF=6.048]Takabayashi T et al. Increased expression of L‐plastin in nasal polyp of patients with nonsteroidal anti‐inflammatory drug exacerbated respiratory disease.(2018) AllergyIHC, Human [IF=4.249]Zha C et al. Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.Neuropharmacology. 2018 Aug;138:140-150.ICF, Human [IF=2.98]Milano, M., et al. "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." Journal of Applied Toxicology 36.1 (2016): 151-160WB, Rat