文献支持

F3 | Factor III/CD142 Rabbit p

Ab(bs-4690R)-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-4690R
更新日期2026年10月13日

10 年钻石会员

详细信息
文献和实验
技术资料

规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-4690R
英文名称	Tissue factor Rabbit pAb
中文名称	组织因子（CD142）抗体
英文别名	CD 142; CD142; CD142 antigen; Coagulation factor III(thromboplastin tissue factor); Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor; TF_HUMAN.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit, GuineaPig)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human Tissue factor
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	29 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cardiovascular > Angiogenesis > Angiogenic Factors Cardiovascular > Blood > Coagulation > Common Cardiovascular > Blood > Coagulation > Extrinsic Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > CD markers ELISA kits
亚基	Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.
亚细胞定位	Isoform 1: Membrane; Single-pass type I membrane protein. _x000D_ Isoform 2: Secreted.
组织特异性	Lung, placenta and pancreas.
相似性	Belongs to the tissue factor family.
功能	Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}

Tissue/cell: rat pancreas tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated(bs-4690R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_

P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Polyclonal Antibody, Unconjugated (bs-4690R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sample:
_x000D_ Lane 1: Mouse Lung tissue lysates
_x000D_ Lane 2: Mouse Placenta tissue lysates
_x000D_ Lane 3: Human A431 cell lysates
_x000D_ Lane 4: Human MDA-MB-231 cell lysates
_x000D_ Lane 5: Human SiHa cell lysates
_x000D_ Primary: Anti-Tissue factor (bs-4690R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 29 kDa
_x000D_ Observed band size: 50 kDa

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。