Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5-HT Polyclonal Antibody, Unconjugated(bs-1126R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-1126R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1126R |
| 英文名称 | 5-HT Rabbit pAb |
| 中文名称 | 5羟色胺抗体 |
| 英文别名 | Serotonin; 5-hydroxytryptamine; 3-(2-Aminoethyl)-5-hydroxyindole hydrochloride; 5 hydroxy tryptophan; 5 Hydroxytryptophan; 5HTP; 5-HT, 5-Hydroxytryptamine hydrochloride. |
| 中文别名 | 5-羟色胺; 五羟色胺; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated 5-HT |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 0.21268 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmitter > Biogenic Amines > Serotonin / 5HT |
| 功能 | Tryptophan is an amino acid occurring naturally in food. In the brain, it is converted to 5-hydroxy-tryptophan (5-HTP), which, in turn, is converted to serotonin. Serotonin is a neurotransmitter essential to regulating appetite, sleep, mood and pain levels. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Serotonin (pronounced) (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system (CNS) and enterochromaffin cells in the gastrointestinal tract of animals including humans. Serotonin is also found in many mushrooms and plants, including fruits and vegetables. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5-HT Polyclonal Antibody, Unconjugated(bs-1126R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5-HT Polyclonal Antibody, Unconjugated(bs-1126R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验该产品被引用文献
[IF={{ 8.2 }}] {Yang Ding. et al. Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis. INT J BIOL SCI. 2024 Aug;20(11):4476-4495} {IF} {Mouse,Human}
[IF={{ 5.81 }}] {Shengyan Xi. et al. The Ciji-Hua’ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma. Front Pharmacol. 2021; 12: 715824} {IHC} {Mouse}
[IF={{ 5.2 }}] {Liu Qihong. et al. Effects of Liqi Tongbian decoction on gut microbiota, SCFAs production, and 5-HT pathway in STC rats with Qi Stagnation Pattern. FRONT MICROBIOL. 2024 Mar;15:} {IHC} {Rat}
[IF={{ 4.2 }}] {Ding Yang. et al. Early-Life Stress Induced by Neonatal Maternal Separation Leads to Intestinal 5-HT Accumulation and Causes Intestinal Dysfunction. J INFLAMM RES. 2024 Nov 18} {IF} {Mouse}
[IF={{ 3.5 }}] {Hongbo Jiang. et al.Effects of aminooxyacetic acid on learning and memory function and neurochemical changes in chronic alcoholism.brain research bulletin.2025 Feb:221:111203.} {IHC} {Rat}
相关实验
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
rabbit serum albumin 兔血清白蛋白 R-Sc Reed-Sternberg's cell 瑞-司氏细胞 Z/R Reinheit Zahl 光密度比值 SAV small agranular vesicle 无颗粒小泡 SBA soybean agglutinin 大豆凝集素 SC secretory component 分泌成份 SCV small clear vesicle 清亮小泡 SDS sodium
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5-HT Rabbit pAb(bs-1126R)-50ul/100ul/200ul
￥1180 - 2800
