Tissue/cell: rat spleen tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-PAFAH2 Polyclonal Antibody, Unconjugated(bs-1451R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
_x000D_
- ￥1180 - 2800
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- bs-1451R
- 2026年10月13日
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- 文献和实验
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1451R |
| 英文名称 | PLA2G7 Rabbit pAb |
| 中文名称 | 脂蛋白磷脂酶A2抗体 |
| 英文别名 | Lp-PLA2; PAFAH; lipoprotein-associated phospholipase A2; Platelet-activating factor acetylhydrolase; Lipoprotein-associated phospholipaseA2(Lp-PLA2); PAF acetylhydrolase; PAF 2-acylhydrolase; LDL-associated phospholipase A2; LDL-PLA(2); 2-acetyl-1-alkylglycerophosphocholine esterase; 1-alkyl-2-acetylglycerophosphocholine esterase; PAFA_HUMAN. |
| 中文别名 | 脂蛋白相关磷脂酶A2; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PAFAH |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood Cardiovascular > Blood > Platelets Immunology > Innate Immunity > Macrophage / Inflamm. Immunology > Secreted Molecules > Other secreted molecules |
| 亚细胞定位 | Secreted, extracellular space. |
| 组织特异性 | Plasma._x000D_ |
| 相似性 | Belongs to the AB hydrolase superfamily. Lipase family. |
| 功能 | Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl -sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-PAFAH2/Lp-PLA2 Polyclonal Antibody, Unconjugated(bs-1451R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-PAFAH2/Lp-PLA2 Polyclonal Antibody, Unconjugated(bs-1451R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFAH) Polyclonal Antibody, Unconjugated (bs-1451R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFAH) Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ Lung (Rat) Lysate at 40 ug
_x000D_ Primary: Anti-PAFAH (bs-1451R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 48 kD
_x000D_ Observed band size: 48 kD
_x000D_
_x000D_ Lung (Rat) Lysate at 40 ug
_x000D_ Primary: Anti-PAFAH (bs-1451R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 48 kD
_x000D_ Observed band size: 48 kD
_x000D_
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文献和实验该产品被引用文献
[IF=4.8] Yangyang Tao. et al. Melatonin suppresses atherosclerosis by ferroptosis inhibition via activating NRF2 pathway. FASEB J. 2024 May;38(10):e23678 WB,IF Mouse
[IF=3.71] Rosing, Katharina, et al. Everolimus therapy is associated with reduced lipoprotein-associated phospholipase A2 (Lp-Pla2) activity and oxidative stress in heart transplant recipients. Atherosclerosis. 2013 Sep;230(1):164-70. WB ="Human"
[IF=3.6] Zhiyu Li. et al. Metformin suppresses metabolic dysfunction-associated fatty liver disease by ferroptosis and apoptosis via activation of oxidative stress. FREE RADICAL RES. 2024 Oct 18 IF,WB Human
[IF=2.93] Wang, Gangqi, et al. "Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NF [kgr] B pathways." Scientific Reports 4 (2014). WB ="Human"
[IF=2.1] Han et al. Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFκB pathways. (2015) Biol.Open. 4:661-5 WB Porcine
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Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
is considered a chronic inflammatory condition of the vessel wall and involves a high chronic concentration of low?density lipoprotein (LDL) in blood. In humans, restenosis develops after intravascular interventions such as angioplasty and stent placement
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PLA2G7 | LDL-PLA2 Rabbit pAb(bs-1451R)-50ul/100ul/200ul
￥1180 - 2800
