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CD68 Mouse mAb(bsm-33056M)-50u

l/100ul
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  • ￥1180 - 1980
  • Bioss已认证
  • bsm-33056M
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    产品编号bsm-33056M
    英文名称CD68 Mouse mAb
    中文名称CD68单克隆抗体
    英文别名CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68(microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP4.
    产品应用IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat, spleen, spleen, spleen, spleen, human lung carcinoma, human lung carcinoma, human lung carcinoma, human lung carcinoma, lymphoid, lymphoid, lymphoid, lymphoid, human tonsil, human tonsil, human tonsil, human tonsil, 肝, 肝, 肝, 肝, spleen, spleen, spleen, Human diffuse large B-cell lymphoma, Human diffuse large B-cell lymphoma, Human diffuse large B-cell lymphoma, Human diffuse large B-cell lymphoma
    抗体来源Mouse
    免疫原Recombinant human CD68 1-100/354aa
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Monoclonal
    理论分子量37 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Immunology > Cell Type Markers > CD > Myeloid Cells

    Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus

    亚细胞定位Isoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
    组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    翻译后修饰N- and O-glycosylated
    相似性Belongs to the LAMP family.
    功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {IHC-P}{1:50-200}
    {IHC-F}{1:50-200}
    {IF}{1:50-200}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (Human diffuse large B-cell lymphoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD68 Monoclonal Antibody, Unconjugated (bsm-33056M) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

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    该产品被引用文献

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    [IF={{ 3.7 }}] {Sun Xuri. et al. Exogenous NT-3 Promotes Phenotype Switch of Resident Macrophages and Improves Sciatic Nerve Injury through AMPK/NF-κB Signaling Pathway. NEUROCHEM RES. 2024 Jun;:1-15} {IF} {Rat}

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    文献支持
    CD68 Mouse mAb(bsm-33056M)-50ul/100ul
    ￥1180 - 1980