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产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul/200ug
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 规格: | 200ug | 产品价格: | ¥5600.0 |
| 产品编号 | bsm-33053M |
| 英文名称 | AMACR Mouse mAb |
| 中文名称 | α-甲基酰基辅酶A消旋酶单克隆抗体 |
| 英文别名 | AMACR_HUMAN; alpha-Methylacyl-CoA racemase; EC:5.1.99.4; 2-methylacyl-CoA racemase; RM; RACE; CBAS4; P504S; AMACRD; |
| 产品应用 | WB=1:500-1000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human AMACR protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 42 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Proto-oncogenes Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Signal Transduction > Metabolism > Lipid metabolism Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚细胞定位 | Peroxisome. Mitochondrion. |
| 相似性 | Belongs to the CaiB/BaiF CoA-transferase family. |
| 功能 | Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neiGHBoring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |

MCF-7(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- AMACR (bsm-33053M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Liver tissue lysates
Primary: Anti- AMACR (bsm-33053M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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文献和实验T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
. Use gloves and aerosol barrier tips. Linearize the plasmid and check the digest. Phenol extract. Extract twice with chloroform:isoamyl alcohol (24:1) Ethanol precipitate ( 1/2 vol 7.5 M NH4 OAc + 2.5 vol 100
Hybrid Resistance by Mouse NK Cells In Vitro
A purified NK cells to kill H2d target cells can be reversed by adding MAb reagents that bind to either Ly49A or H2-Dd . We and others have extended these early findings to the Ly49C and I subsets of NK cells, and have recently detected positive signaling
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