Sample:
_x000D_
Lane 1: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
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Lane 2: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
_x000D_
Primary: Anti-TAP-Tag (bsm-33133M) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
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Predicted band size: 51 kD
_x000D_
Observed band size: 51 kD
- ￥980 - 5600
- Bioss
- bsm-33133M
- 2026年10月13日
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- 文献和实验
- 技术资料
- 规格：
100ul/500ul/200ug
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-33133M |
| 英文名称 | TAP-Tag Mouse mAb |
| 中文名称 | TAP-Tag（标签）单克隆抗体 |
| 英文别名 | TAP Tag; TAPTag; Tandem affinity purification |
| 产品应用 | WB=1:1000-5000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant TAP-Tag |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {ELISA} | {1:1000-5000} |
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抗凝血酶III（Antithrombin，AT-III）的生理功能与临床应用
来源 : Human 表达宿主 : HEK293 标记 : Unconjugated UA010467 SerpinC1/Antithrombin III His Tag Protein, Mouse 来源 : Mouse 表达宿主 : HEK293 标记 : Unconjugated UA010440 SerpinC1/Antithrombin III His Tag Protein, Human 来源 : Human 表达宿主 : HEK293 标记
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TAP Tag Mouse mAb(bsm-33133M)-100ul/500ul/200ug
￥980 - 5600
