Sample:
Hela (humsn) Cell Lysate at 40 ug
HepG2 (human) Cell Lysate at 40 ug
Primary: Anti-PEPD (bs-12616R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
- ￥1180 - 2800
- Bioss
- bs-12616R
- 2025年10月24日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12616R |
| 英文名称 | PEPD Rabbit pAb |
| 中文名称 | 脯氨酸二肽酶抗体 |
| 英文别名 | Aminoacyl L proline hydrolase; Imidodipeptidase; MGC10905; MGC95081; Pep 4; Pep4; pepD; PEPD_HUMAN; Peptidase 4; Peptidase D; Prolidase; Proline dipeptidase; X pro dipeptidase; X-Pro dipeptidase; Xaa-Pro dipeptidase. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Horse, Zebrafish) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PEPD |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Signal Transduction > Metabolism > Amino Acids |
| 亚基 | Interacts with TRAF6, IRAK1, IRAK4 and MAP3K7. Interacts with BCL10; this interaction is impaired by SOCS3 (By similarity). |
| 翻译后修饰 | Phosphorylated by IRAK1 and IRAK4 enhancing its E3 ligase activity. |
| 相似性 | Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. |
| 功能 | Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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PEPD Rabbit pAb(bs-12616R)-50ul/100ul/200ul
￥1180 - 2800
