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- Bioss
- bs-19774R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-19774R |
| 英文名称 | RBM47 Rabbit pAb |
| 中文名称 | RNA结合蛋白47抗体 |
| 英文别名 | NET18; Rbm47; Rbm47 RNA binding motif protein 47; RBM47_HUMAN; RNA binding motif protein 47; RNA binding protein 47; RNA-binding motif protein 47; RNA-binding protein 47. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RBM47 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 64 , 57 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding |
| 亚细胞定位 | Nucleus. |
| 相似性 | Belongs to the RRM RBM47 family. Contains 3 RRM (RNA recognition motif) domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM47) Polyclonal Antibody, Unconjugated (bs-19774R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-RBM47 (bs-19774R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 , 57 kDa
Observed band size: 62 kDa
Lane 1: Human A431 cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-RBM47 (bs-19774R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 , 57 kDa
Observed band size: 62 kDa
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文献和实验该产品被引用文献
[IF={{ 6.1 }}] {Ma Yihui. et al. RBM47 promotes cell proliferation and immune evasion by upregulating PDIA6: a novel mechanism of pancreatic cancer progression. J TRANSL MED. 2024 Dec;22(1):1-21} {IHC,IF,WB} {Human}
[IF={{ 3.8 }}] {Wei Wei. et al.RBM47 is a novel immunotherapeutic target and prognostic biomarker in gliomas.SCIENTIFIC REPORTS.2025 Jan 5;15(1):854.} {IHC} {Mouse}
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RBM47 Rabbit pAb(bs-19774R)-50ul/100ul/200ul
￥1180 - 2800
