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产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-18609R |
| 英文名称 | MAD3 Rabbit pAb |
| 中文名称 | MAD3蛋白抗体 |
| 英文别名 | bHLHc13; Class C basic helix-loop-helix protein 13; FLJ35523; Likely ortholog of mouse Max dimerization protein 3; MAD3_HUMAN; MAX dimerization protein 3; Max dimerizer 3; Max-associated protein 3; Max-interacting transcriptional repressor MAD3; MGC2383; MXD3; Myx; OTTHUMP00000223596; OTTHUMP00000223598. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, GuineaPig, Cat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MAD3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH / Leucine Zipper |
| 亚细胞定位 | Nucleus. |
| 相似性 | Contains 1 basic helix-loop-helix (bHLH) domain. |
| 功能 | Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the Myc superfamily of basic helix-loop-helix leucine zipper transcriptional regulators. The encoded protein forms a heterodimer with the cofactor MAX which binds specific E-box DNA motifs in the promoters of target genes and regulates their transcription. Disruption of the MAX-MXD3 complex is associated with uncontrolled cell proliferation and tumorigenesis. Transcript variants of this gene encoding different isoforms have been described.[provided by RefSeq, Dec 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

MCF-7(Mouse) Cell Lysate at 30 ug
Primary: Anti-MAD3(bs-18609R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 42 kD
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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