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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-18136R |
| 英文名称 | Lambda Light Chain Rabbit pAb |
| 中文名称 | λ轻链抗体 |
| 英文别名 | IGLC1_HUMAN; Immunoglobulin lambda constant 1; IGLC1; Ig lambda chain C region MGC; Ig lambda-1 chain C region; IGLC; |
| 中文别名 | 免疫球蛋白λ轻链 |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Human Lambda Light Chain |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 11 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Immunoglobulins > Light Chain > Lambda |
| 亚细胞定位 | Cytoplasmic |
| 相似性 | Contains 1 Ig-like (immunoglobulin-like) domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

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文献和实验Generation of Rabbit Immune Libraries
for the combinatorial cloning of the variable heavy and light chain immunoglobulin repertoire of rabbits for the subsequent phage display of rabbit scFv antibody fragments is described.
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
ml of alkaline phosphatase buffer. Soak membrane sections in this solution for 5 - 10 minutes while mixing. Keep this mixture under foil to reduce exposure to light. Library Screening: Once the serum is sufficiently adsorbed, a lambda-gt11
Production of Human Antibodies from Transgenic Mice
light-chain locus has been inactivated by knocking out the Jκ and/or the Cκ genes; 3. the endogenous murine lambda light-chain locus remains intact and functional;
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