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- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3461R |
| 英文名称 | phospho-PPIG (Ser376) Rabbit pAb |
| 中文名称 | 磷酸化亲环蛋白（亲环素）PPIG抗体 |
| 英文别名 | CARS cyclophilin; CARS Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G; Peptidylprolyl isomerase G(cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Rotamase G; RotamaseG; PPIG_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human PPIG around the phosphorylation site of Ser376 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 89 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II. |
| 亚细胞定位 | Nucleus matrix. Nucleus speckle. Note=Colocalizes with RNA splicing factors at nuclear speckles. |
| 组织特异性 | Ubiquitous. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Contains 1 PPIase cyclophilin-type domain. |
| 功能 | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PPIG belongs to the the peptidyl-prolyl cis/trans isomerase protein family (PPIases). PPIases accelerate the folding of proteins. PPIG catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is distributed in nuclear speckles, a nuclear compartment rich in splicing factors, and colocalizes with the splicing factors SC35 and pinin, suggesting a role in the regulation of pre-mRNA splicing. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Testis (Mouse) Lysate at 40 ug
HL60(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-PPIG (Ser376) (bs-3461R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 89 kD
Observed band size: 110 kD
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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