Blank control (blue line): Hela(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Phospho-CEBP beta (Thr235) antibody (bs-3063R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
- ￥1180 - 2800
- Bioss
- bs-3063R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3063R |
| 英文名称 | phospho-CEBP beta (Thr235) Rabbit pAb |
| 中文名称 | 磷酸化转录调节因子CEBP β抗体 |
| 英文别名 | P-CEBP beta(Thr235); P-CEBP beta(T235); CEBP-beta; CLEBP beta; CCAAT Enhancer Binding Protein beta; AGP/EBP; C EBP beta; C/EBP related protein 2; CEBPB; CRP2; IL 6DBP; IL6DBP; Interleukin 6 dependent binding protein; LAP; Liver enriched transcriptional activator; NF IL6; NFIL6; Nuclear factor NF IL6; SF B; SFB; Silencer factor B; TCF5; Transcription factor 5; C/EBP beta; CCAAT/enhancer-binding protein beta; CEBPB_HUMAN; Liver activator protein; Liver enriched transcriptional activator; Nuclear factor NF IL6; Nuclear factor NF-IL6; TCF-5. |
| 产品应用 | Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human CEBP beta around the phosphorylation site of Thr235 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 38 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Heart > Hypertrophy > Transcription factors Epigenetics and Nuclear Signaling > Transcription > Other factors Epigenetics and Nuclear Signaling > Transcription > Transcription Factors |
| 亚基 | Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed at low levels in the lung, kidney and spleen. |
| 翻译后修饰 | Sumoylated by polymeric chains of SUMO2 or SUMO3. |
| 相似性 | Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. |
| 功能 | Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /test} |
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phospho-CEBP beta (Thr235) Rabbit pAb(bs-3063R)-50ul/100ul/200ul
￥1180 - 2800
