Tissue/cell: Human 293FT cell;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SEC61 bata Polyclonal Antibody, Unconjugated(bs-7404R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
- ￥1180 - 2800
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- bs-7404R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7404R |
| 英文名称 | SEC61 beta Rabbit pAb |
| 中文名称 | 转运蛋白SEC61β亚基抗体 |
| 英文别名 | SEC61BProtein translocation complex beta; Protein transport protein SEC61 beta subunit; Protein transport protein Sec61 subunit beta; SEC 61B; SC61B_HUMAN; SEC61; SEC61 B; Sec61 beta subunit; Sec61 complex beta subunit. |
| 产品应用 | ICC/IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SEC61 beta |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 9.8 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Trafficking > ER Proteins |
| 亚基 | Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma. Part of a complex composed of SEC61, SEC62 and SEC63. Interacts with SEC62. |
| 亚细胞定位 | Endoplasmic reticulum membrane. |
| 相似性 | Belongs to the SEC61-beta family. |
| 功能 | Necessary for protein translocation in the endoplasmic reticulum. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
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SEC61 beta Rabbit pAb(bs-7404R)-50ul/100ul/200ul
￥1180 - 2800
