Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HTRA4 Polyclonal Antibody, Unconjugated(bs-18101R) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-18101R
- 2025年10月16日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-18101R |
| 英文名称 | HTRA4 Rabbit pAb |
| 中文名称 | 丝氨酸肽酶4抗体 |
| 英文别名 | FLJ90724; High temperature requirement protean A4; High-temperature requirement factor A4; HTRA 4; HtrA serine peptidase 4; HTRA4; HTRA4_HUMAN; Probable serine protease HTRA4; Serine protease HTRA4. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human HTRA4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the peptidase S1B family. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain. Contains 1 PDZ (DHR) domain. |
| 功能 | Serine protease. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging. [provided by RefSeq, Oct 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Protein: DU145(human) lyates at 30ug;
Primary: Rabbit Anti-HTRA4(bs-18101) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:49 kD
Observed band size:50 kD
Primary: Rabbit Anti-HTRA4(bs-18101) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:49 kD
Observed band size:50 kD
Sample:
PC-3 Cell (Human) Lysate at 30 ug
Primary: Anti- HTRA4 (bs-18101R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
PC-3 Cell (Human) Lysate at 30 ug
Primary: Anti- HTRA4 (bs-18101R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-HTRA4 (bs-18101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-HTRA4 (bs-18101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HTRA4) Polyclonal Antibody, Unconjugated (bs-18101R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 3.8 }}] {Kong Hongfang. et al. Htra4 promotes vascular endothelial cell injury and is associated with the early-onset of preeclampsia. SCI REP-UK. 2025 Apr;15(1):1-11} {WB} {Human}
[IF={{ 2.7 }}] {Yan Chen. et al. HtrA3: a promising prognostic biomarker and therapeutic target for head and neck squamous cell carcinoma. PEERJ. 2023 Oct;11:e16237} {IHC,WB} {Human}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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HTRA4 Rabbit pAb(bs-18101R)-50ul/100ul/200ul
￥1180 - 2800
