Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAM1 Polyclonal Antibody, Unconjugated(bs-16565R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-16565R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16565R |
| 英文名称 | TRAM1 Rabbit pAb |
| 中文名称 | 相关易位膜蛋白1抗体 |
| 英文别名 | PNAS 8; PNAS8; PRO1292; TRAM 1; TRAM; TRAM1_HUMAN; TRAMP; Translocating chain associated membrane protein 1; Translocating chain associating membrane protein; Translocation associated membrane protein 1; Translocation associating membrane protein 1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TRAM1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Trafficking > Organelle Proteins Tags & Cell Markers > Subcellular Markers > Organelles > ER |
| 亚基 | Interacts with human cytomegalovirus/HHV-5 protein US2. |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multipass membrane protein |
| 相似性 | Belongs to the TRAM family. Contains 1 TLC (TRAM/LAG1/CLN8) domain. |
| 功能 | Stimulatory or required for the translocation of secretory proteins across the ER membrane. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | TRAM1 belongs to the TRAM family. It stimulates or is required for the translocation of secretory proteins across the endoplasmic reticulum membrane. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验相关实验
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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TRAM1 Rabbit pAb(bs-16565R)-50ul/100ul/200ul
￥1180 - 2800
