Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-HAPLN3 antibody (bs-15411R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-15411R
- 2025年10月24日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-15411R |
| 英文名称 | HAPLN3 Rabbit pAb |
| 中文名称 | 透明质酸及粘蛋白3抗体 |
| 英文别名 | EXLD1; Extracellular link domain containing, 1; HAPLN3; HPLN3_HUMAN; HsT19883; Hyaluronan and proteoglycan link protein 3. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Pig, Horse, Chimpanzee) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human HAPLN3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix (By similarity). |
| 组织特异性 | Widely expressed with highest levels in spleen and placenta. |
| 相似性 | Belongs to the HAPLN family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains |
| 功能 | May function in hyaluronic acid binding. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | HAPLN3 is a 360 amino acid protein encoded by the human gene HAPLN3. HAPLN3 belongs to the HAPLN family and contains 1 Ig-like V-type (immunoglobulin-like) domain and two Link domains. HAPLN3 mediates the binding of complexes containing hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. HAPLN3 may also be involved in the formation of extracellular matrices contributing to perineuronal nets and facilitate the understanding of a functional role of these extracellular matrices. HAPLN3 is widely expressed with highest levels in spleen and placenta. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
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HAPLN3 Rabbit pAb(bs-15411R)-50ul/100ul/200ul
￥1180 - 2800
