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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-7894R |
| 英文名称 | CDCA3 Rabbit pAb |
| 中文名称 | 细胞分裂周期相关蛋白3抗体 |
| 英文别名 | CDCA 3; Cell division cycle associated 3; Cell division cycle associated protein 3; Gene rich cluster C8; Gene rich cluster protein C8; GRCC 8; GRCC8; MGC2577; TOME 1; TOME1; Trigger of mitotic entry 1; C8 antibody; Trigger of mitotic entry protein 1; CDCA3_HUMAN . |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CDCA3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Kinases/Phosphatases > Cdk Regulators Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ubiquitin E3 Enzymes > SCF Complex E3 Ligase Epigenetics and Nuclear Signaling > Cell cycle > Kinases/Phosphatases > Cdk Regulators Epigenetics and Nuclear Signaling > Cell cycle > Ubiquitin ligases |
| 亚基 | Protein modification; protein ubiquitination. [SUBUNIT] Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). |
| 亚细胞定位 | Cytoplasm, cytosol (By similarity). |
| 翻译后修饰 | Ubiquitinated and degraded by the APC/C-Cdh1 complex (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 功能 | F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 5.7 }}] {Zhou Mingxuan. et al. Identification of immune-related genes and small-molecule drugs in hypertension-induced left ventricular hypertrophy based on machine learning algorithms and molecular docking. FRONT IMMUNOL. 2024 Jun;15:} {IF,WB} {Mouse}
[IF={{ 4.8 }}] {Daolin Ji. et al. The functional roles of deoxyelephantopin potential target circTNPO3 in regulating pancreatic cancer malignant phenotype and gemcitabine chemoresistance via miR-188-5p/CDCA3/TRAF2-mediated remodeling of NF-κB signaling pathway. FRONT PHARMACOL. 2025 Jul;16:1613560} {WB} {Human}
[IF={{ 3.58 }}] {Wang, Qi, et al. "Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies." Food and Chemical Toxicology (2017).} {WB} {="Rat"}
[IF={{ 2.9 }}] {Wei Ding. et al. Roles of the CDCA gene family in breast carcinoma. SCI PROGRESS-UK. ;():} {WB,IHC} {Human}
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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