Blank control: Mouse Liver Cells(fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti- MGLUR3/PE Conjugated antibody (bs-12012R-PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.
- ￥1180 - 2800
- Bioss
- bs-12012R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12012R |
| 英文名称 | MGLUR3 Rabbit pAb |
| 中文名称 | 代谢型谷氨酸受体-3抗体 |
| 英文别名 | G protein coupled receptor family C group 1 member C; GLUR 3; GLUR3; GLUR3; Glutamate metabotropic receptor 3; Glutamate receptor metabotropic 3; GPRC1C; GRM 3; GRM3; GRM3_HUMAN; Metabotropic glutamate receptor 3; Metabotropic glutamate receptor 3 precursor; mGlu 3; MGlu3; MGlu3; MGLUR 3; MGLUR3. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=5μg/Test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Metabotropic Glutamate Receptor 3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 96 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Glutamate Receptors Neuroscience > Neurotransmitter > Amino Acids > Glutamate Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚基 | nteracts with GRASP |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 相似性 | Belongs to the G-protein coupled receptor 3 family. |
| 功能 | Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {5μg/Test} |
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (bs-12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (bs-12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (bs-12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (bs-12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (bs-12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (bs-12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
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文献和实验该产品被引用文献
[IF={{ 2.666 }}] {X Zhou et al. Modulating NMDA receptors to treat MK-801-induced schizophrenic cognition deficit: effects of clozapine combining with PQQ treatment and possible mechanisms of action. BMC Psychiatry. 2020 Mar 6;20(1):106.} {WB} {rat}
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MGLUR3 Rabbit pAb(bs-12012R)-50ul/100ul/200ul
￥1180 - 2800
