Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CABC1 Polyclonal Antibody, Unconjugated(bs-8071R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-8071R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8071R |
| 英文名称 | COQ8A Rabbit pAb |
| 中文名称 | 伴侣蛋白bc1同源复合体抗体 |
| 英文别名 | mitochondrial; aarF domain containing protein kinase 3; aarF domain-containing protein kinase 3; ADCK 3; ADCK3; ADCK3_HUMAN; CABC 1; Chaperone ABC1(activity of bc1 complex S.pombe) like; Chaperone ABC1 activity of bc1 complex homolog; Chaperone ABC1 like; Chaperone activity of bc1 complex like; Chaperone activity of bc1 complex like mitochondrial; Chaperone activity of bc1 complex-like; Chaperone-ABC1-like; Coenzyme Q8 homolog; COQ 8; COQ8. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human COQ8A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 72 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Signal Transduction > Protein Trafficking > Chaperones > Other Chaperones Tags & Cell Markers > Subcellular Markers > Organelles > Mitochondria |
| 亚细胞定位 | Mitochondrion. |
| 组织特异性 | Ubiquitously expressed with a relativelygreater abundance in heart and skeletal muscle. |
| 相似性 | Belongs to the protein kinase superfamily. ADCKprotein kinase family. Contains 1 protein kinase domain. |
| 功能 | May be a chaperone-like protein essential for the properconformation and functioning of protein complexes in therespiratory chain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a mitochondrial protein similar to yeast ABC1, which functions in an electron-transferring membrane protein complex in the respiratory chain. It is not related to the family of ABC transporter proteins. Expression of this gene is induced by the tumor suppressor p53 and in response to DNA damage, and inhibiting its expression partially suppresses p53-induced apoptosis. Alternatively spliced transcript variants have been found; however, their full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- CABC1 (bs-8071R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 75 kD
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- CABC1 (bs-8071R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 75 kD
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COQ8A Rabbit pAb(bs-8071R)-50ul/100ul/200ul
￥1180 - 2800
