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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6700R |
| 英文名称 | SRD5A2 Rabbit pAb |
| 中文名称 | 类固醇5α还原酶2抗体 |
| 英文别名 | 5 alpha SR2; EC; SR type 2; Steroid 5 alpha reductase 2; 3 oxo 5 alpha steroid 4 dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; 5ART2; e II 5 alpha reductase; EC; MGC138457; Micropenis, included; S5A2_HUMAN; S5AR 2; SR type 2; SRD5A2; Steroid 5 alpha reductase 2; Steroid 5-alpha-reductase 2; Steroid-5-alpha-reductase, alpha polypeptide 2(3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2); Type II 5-alpha reductase. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Pig, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SRD5A2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 28 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell differentiation Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Vitamins / minerals Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Vitamins / Minerals Signal Transduction > Protein Trafficking > Organelle Proteins |
| 亚细胞定位 | Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). |
| 组织特异性 | Expressed in high levels in the prostate and many other androgen-sensitive tissues. |
| 相似性 | Belongs to the steroid 5-alpha reductase family. |
| 功能 | Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 8.2 }}] {Qingjing Gao. et al. 1,25(OH)₂D₃ regulates androgen synthesis via transcriptional control of steroidogenic enzymes and LHR in the scented glands of muskrats (Ondatra zibethicus). FREE RADICAL BIO MED. 2025 Mar;229:82} {IHC} {Muskrat}
[IF={{ 4.522 }}] {Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019 Jan 22.} {WB} {Sheep}
[IF={{ 4.292 }}] {Longfei Xiao. et al. Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. J Steroid Biochem. 2021 Nov;214:106001} {WB,IHC} {Bovine}
[IF={{ 4.292 }}] {Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-β/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041} {WB} {Dog}
[IF={{ 4.24 }}] {Tanaka, Sota, et al. "The role of 5α-reductase type 1 associated with intratumoral dihydrotestosterone concentrations in human endometrial carcinoma." Molecular and Cellular Endocrinology (2014).} {IHC} {="Human"}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Phenotypic Analysis of BAT versus WAT Differentiation
Primer pairs for designated housekeeping (see below) and target genes (Table 13.1.6700 ) Tissue homogenizer
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