Sample:Lymph node (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
- ￥1180 - 2800
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- bs-5973R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5973R |
| 英文名称 | SIRT7 Rabbit pAb |
| 中文名称 | 沉默调节样蛋白SirT7抗体 |
| 英文别名 | NAD dependent deacetylase sirtuin 7; NAD-dependent deacetylase sirtuin-7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIRT 7; SIR7_HUMAN; Sirtuin 7; Sirtuin type 7; Sirtuin7. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Dog, Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SIRT7 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Transcription factors Cardiovascular > Atherosclerosis > Apoptosis Cardiovascular > Heart > Cardiac metabolism Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Acetylation > HDACs > Class III / Sir2 class Epigenetics and Nuclear Signaling > Transcription > Co-factors Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol I Transcription Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Co-factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Nucleotide metabolism > Purine and pyrimidine synthesis Metabolism > Types of disease > Heart disease |
| 亚基 | Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B. |
| 亚细胞定位 | Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis. |
| 翻译后修饰 | Phosphorylated during mitosis (Probable). |
| 相似性 | Belongs to the sirtuin family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain. |
| 功能 | NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(bs-5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(bs-5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:Small intestine (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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文献和实验该产品被引用文献
[IF={{ 3.06 }}] {Wronska, A., et al. "Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats." Gerontology (2015).} {WB} {=""}
[IF={{ 2.65 }}] {Deng Disi. et al. Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide. EVID-BASED COMPL ALT. 2022;2022:3642349} {IHC} {Rat}
[IF={{ 1.11 }}] {Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8.} {IHC} {="Mouse"}
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SIRT7 Rabbit pAb(bs-5973R)-50ul/100ul/200ul
￥1180 - 2800
