Sample: U251 Cell lysate at 30ug;
Primary: Anti-Phospho-Tyrosine Hydroxylase (Ser40) (bs-3462R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 60 kD
Observed band size: 60 kD
- ￥1180 - 2800
- Bioss
- bs-3462R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3462R |
| 英文名称 | phospho-Tyrosine Hydroxylase (Ser40) Rabbit pAb |
| 中文名称 | 磷酸化酪氨酸羟化酶抗体 |
| 英文别名 | Tyrosine Hydroxylase(phospho-Ser40); Tyrosine Hydroxylase; DYT14; DYT5b; ple; Protein Pale; TH; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; TY3H_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from rat TH around the phosphorylation site of Ser40 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Cancer > Cancer Metabolism > Response to hypoxia Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Neuroscience > Cell Type Marker > Neuron marker > Soma marker Neuroscience > Neurotransmitter > Biogenic Amines > Dopamine Neuroscience > Neurotransmitter > Biogenic Amines > Noradrenaline |
| 亚基 | Homotetramer. |
| 亚细胞定位 | Mainly expressed in the brain and adrenal glands. |
| 组织特异性 | Mainly expressed in the brain and adrenal glands. |
| 翻译后修饰 | In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. |
| 相似性 | Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. |
| 功能 | Plays an important role in the physiology of adrenergic neurons. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-0016P is one synthetic peptide derived from human TH. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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phospho-Tyrosine Hydroxylase (Ser40) Rabbit pAb(bs-3462R)-50ul/100ul/200ul
￥1180 - 2800
