Sample:
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293T Cell (Human) Lysate at 40 ug
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293FT Cell (Human) Lysate at 40 ug
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Primary: Anti-FAPP2 (bs-16026R) at 1/300 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 58 kD
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Observed band size: 58 kD
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- ￥1180 - 2800
- Bioss
- bs-16026R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16026R |
| 英文名称 | FAPP2 Rabbit pAb |
| 中文名称 | FAPP2蛋白抗体 |
| 英文别名 | FAPP-2; FAPP2; hFAPP2; MGC3358; PH domain-containing family A member 8; Phosphatidylinositol-four-phosphate adapter protein 2; phosphoinositide binding specificmember 8; phosphoinositol 4 phosphate adaptor protein 2; phosphoinositol 4-phosphate adapter protein 2; PKHA8_HUMAN; Pleckstrin homology domain containing family A(phosphoinositide binding specific) member 8; Pleckstrin homology domain containing family A; Pleckstrin homology domain-containing family A member 8; Plekha8; PLEKHA8 protein; Serologically defined breast cancer antigen NY-BR-86. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FAPP2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Metabolism > Lipid metabolism Signal Transduction > Protein Trafficking > Chaperones > Other Chaperones |
| 亚细胞定位 | Cytoplasm. Localized to perinuclear structures in the trans-Golgi network. |
| 组织特异性 | Expressed in kidney cell lines. |
| 相似性 | Contains 1 PH domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Involved in TGN-to-plasma membrane transport and in the formation of post-Golgi constitutive carriers. May play a role in ensuring the coordination of the budding and the fission reactions. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FAPP2 Polyclonal Antibody, Unconjugated(bs-16026R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FAPP2 Polyclonal Antibody, Unconjugated(bs-16026R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
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文献和实验该产品被引用文献
[IF=14.808] Noritaka Saeki. et al. Epigenetic regulator UHRF1 suppressively orchestrates pro-inflammatory gene expression in rheumatoid arthritis. J CLIN INVEST. 2022 Apr;: IF Human
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PLEKHA8 | FAPP2 Rabbit pAb(bs-16026R)-50ul/100ul/200ul
￥1180 - 2800
