Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Placental lactogen I+II Polyclonal Antibody, Unconjugated(bs-12940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-12940R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12940R |
| 英文名称 | Placental lactogen I+II Rabbit pAb |
| 中文名称 | 胎盘催乳素1/2抗体 |
| 英文别名 | Choriomammotropin; Chorionic somatomammotropin B; Chorionic somatomammotropin hormone 2; Chorionic somatomammotropin hormone; CS 2; CS2; CSB; CSH_HUMAN; CSH1; CSH2; hCS B; HCSB; Lactogen; mPL II; mplII; Pl 2; PL; Pl2; Placental lactogen; Placental lactogen II; Prl3b1; Prolactin 3B1; prolactin family 3, subfamily b, member 1; Placental lactogen I; Placental lactogen II; rPLII. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CSH2/Placental lactogen I/II |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Reproduction > Hormones Signal Transduction > Growth Factors/Hormones > Hormones |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. |
| 相似性 | Belongs to the somatotropin/prolactin family. |
| 功能 | Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Placental lactogens, also referred to as chorionic somatomammotropin hormones, are protein hormones. They are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus. Placental lactogen I is a member of the somatotropin/prolactin family of hormones. The proteins in this family are crucial in mammalian growth control. Placental lactogen I is expressed primarily during mid-pregnancy, and it has been reported that DNA methylation regulates its tissue expression in rats. Placental lactogen II is expressed later in pregnancy and, in mice, its secretion is regulated by the inhibitory control of GH, the concentration of which increases rapidly at the beginning of the last half of pregnancy. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Placental lactogen I+II Polyclonal Antibody, Unconjugated(bs-12940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Placental lactogen I+II Polyclonal Antibody, Unconjugated(bs-12940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Placental lactogen I+II Polyclonal Antibody, Unconjugated(bs-12940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Placental lactogen I+II Polyclonal Antibody, Unconjugated(bs-12940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验相关实验
Bacterial Expression of Prolactin Family Proteins
Complementary DNAs of three recombinant proteins related to the prolactin family: ovine placental lactogen (oPL), ovine prolactin (oPRL), and rabbit soluble extracellular domain of prolactin receptor (rbPRLR-ECD) were subcloned by different
Immunohistochemistry and Immunocytochemistry
1 receptors (AT 1 -R) in the ovine placental artery from a representative ewe at 142 days’ gestation. Dark cytoplasmic color indicates the positive staining. Dark nuclear color is hematoxylin counterstaining. Tissue sections were incubated with mouse antibody
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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Placental lactogen I+II Rabbit pAb(bs-12940R)-50ul/100ul/200ul
￥1180 - 2800
