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—20℃,12个月
2年
20
康朗生物
pET-46 EK/LIC
5ug
质粒类型: | 大肠杆菌表达载体 |
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表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5200 bp (查看载体序列) |
5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
载体标签: | N-His, N-EK, C-S |
载体抗性: | Ampicillin (氨苄青霉素) |
备注: |
For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
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产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
KL1938 | pET-46 EK/LIC | 5ug质粒 |
询价 |
The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Fusion proteins contain an N-terminal cleavable His•Tag® sequence. Fusion to an optional C-terminal S•TagTM sequence can also be created for detection, purification and quantification of fusion proteins. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 Terminator Primer (Cat. No. 69337-3).
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