质粒编号: 质粒名称: pGPTV-DHFR 其他名称: 质粒大小(bp): 13800 抗性: 质粒类型: Unspecified 是否病毒: 启动子: 稳转或瞬转: 组成型或诱导性: 表达水平: 蛋白标签: 测序引物: GenBank号: 其他属性: Deposited by: Robert Masterson, D. Becker Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced. [1] The order of the major features of this plasmid is: EcoRI – pAnos – SstI – uidA – SmaI – XbaI – SalI – HindIII – Pnos – BglII – dhfr – BamHI – pAg7 – T-DNA – BglII – RK2 ori – kanR. [1] Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI–1.6; EcoRI–11.6; SstI–0.9, 11.9. [1] Restriction digests of the clone give the following sizes (kb): EcoRI–13.5; BglII–8.4, 3.1, 2.3; HindIII–13.5. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli, E.coli HB101, Agrobacterium tumefaciens, higher plants, Agrobacterium tumefaciens LBA4404. 质粒图谱: