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低温运输,-20℃保存
1年
pGEX-KN
大量
信裕生物
名称 | 编号 | 规格 | 价格 | 手册 |
---|---|---|---|---|
pGEX-KN |
质粒编号:
质粒名称: pGEX-KN
其他名称:
质粒大小(bp): 4966
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Deposited by: David J. Hakes, Jack E. Dixon The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [1] Expression vector for rapid purification of fusion proteins that contain no amino terminal extensions after thrombin cleavage. The amino acid after the initiator methionine must be charged. [1] Cloning into this vector requires amplification of the gene using oligonucleotides prepared as in the reference and encoding the first 4 amino acids of a thrombin recognition sequence. [1] Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine kinker and a NotI site. [1] The order of the major features in this plasmid is: pMB1 ori – lacIV – lacZ – tac promoter – GST – glycine kinker – NotI/MCS/EcoRI – ampR. [1] Restriction digests of the clone give the following sizes (kb): NotI–5.0; EcoRI/PstI–4.0, 1.0; BamHI/EcoRV–3.2, 1.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM101, E.coli TG1, E.coli BL21. Related vectors: pGEX-1, pGEX-KT, pGEX-KG.
质粒图谱:
质粒序列:
1 | agcttatcga | ctgcacggtg | caccaatgct | tctggcgtca | ggcagccatc | 50 |
51 | ggaagctgtg | gtatggctgt | gcaggtcgta | aatcactgca | taattcgtgt | 100 |
101 | cgctcaaggc | gcactcccgt | tctggataat | gttttttgcg | ccgacatcat | 150 |
151 | aacggttctg | gcaaatattc | tgaaatgagc | tgttgacaat | taatcatcgg | 200 |
201 | ctcgtataat | gtgtggaatt | gtgagcggat | aacaatttca | cacaggaaac | 250 |
251 | agtattcatg | tcccctatac | taggttattg | gaaaattaag | ggccttgtgc | 300 |
301 | aacccactcg | acttcttttg | gaatatcttg | aagaaaaata | tgaagagcat | 350 |
351 | ttgtatgagc | gcgatgaagg | tgataaatgg | cgaaacaaaa | agtttgaatt | 400 |
401 | gggtttggag | tttcccaatc | ttccttatta | tattgatggt | gatgttaaat | 450 |
451 | taacacagtc | tatggccatc | atacgttata | tagctgacaa | gcacaacatg | 500 |
501 | ttgggtggtt | gtccaaaaga | gcgtgcagag | atttcaatgc | ttgaaggagc | 550 |
551 | ggttttggat | attagatacg | gtgtttcgag | aattgcatat | agtaaagact | 600 |
601 | ttgaaactct | caaagttgat | tttcttagca | agctacctga | aatgctgaaa | 650 |
651 | atgttcgaag | atcgtttatg | tcataaaaca | tatttaaatg | gtgatcatgt | 700 |
701 | aacccatcct | gacttcatgt | tgtatgacgc | tcttgatgtt | gttttataca | 750 |
751 | tggacccaat | gtgcctggat | gcgttcccaa | aattagtttg | ttttaaaaaa | 800 |
801 | cgtattgaag | ctatcccaca | aattgataag | tacttgaaat | ccagcaagta | 850 |
851 | tatagcatgg | cctttgcagg | gctggcaagc | cacgtttggt | ggtggcgacc | 900 |
901 | atcctccaaa | atcggatctg | ggtggtggtg | gcggccgcgg | atccccggga | 950 |
951 | attcatcgtg | actgactgac | gatctgcctc | gcgcgcctcg | cgcgtttcgg | 1000 |
1001 | tgatgacggt | gaaaacctct | gacacatgca | gctcccggag | acggtcacag | 1050 |
1051 | cttgtctgta | agcggatgcc | gggagcagac | aagcccgtca | gggcgcgtca | 1100 |
1101 | gcgggtgttg | gcgggtgtcg | gggcgcagcc | atgacccagt | cacgtagcga | 1150 |
1151 | tagcggagtg | tataattctt | gaagacgaaa | gggcctcgtg | atacgcctat | 1200 |
1201 | ttttataggt | taatgtcatg | ataataatgg | tttcttagac | gtcaggtggc | 1250 |
1251 | acttttcggg | gaaatgtgcg | cggaacccct | atttgtttat | ttttctaaat | 1300 |
1301 | acattcaaat | atgtatccgc | tcatgagaca | ataaccctga | taaatgcttc | 1350 |
1351 | aataatattg | aaaaaggaag | agtatgagta | ttcaacattt | ccgtgtcgcc | 1400 |
1401 | cttattccct | tttttgcggc | attttgcctt | cctgtttttg | ctcacccaga | 1450 |
1451 | aacgctggtg | aaagtaaaag | atgctgaaga | tcagttgggt | gcacgagtgg | 1500 |
1501 | gttacatcga | actggatctc | aacagcggta | agatccttga | gagttttcgc | 1550 |
1551 | cccgaagaac | gttttccaat | gatgagcact | tttaaagttc | tgctatgtgg | 1600 |
1601 | cgcggtatta | tcccgtgttg | acgccgggca | agagcaactc | ggtcgccgca | 1650 |
1651 | tacactattc | tcagaatgac | ttggttgagt | actcaccagt | cacagaaaag | 1700 |
1701 | catcttacgg | atggcatgac | agtaagagaa | ttatgcagtg | ctgccataac | 1750 |
1751 | catgagtgat | aacactgcgg | ccaacttact | tctgacaacg | atcggaggac | 1800 |
1801 | cgaaggagct | aaccgctttt | ttgcacaaca | tgggggatca | tgtaactcgc | 1850 |
1851 | cttgatcgtt | gggaaccgga | gctgaatgaa | gccataccaa | acgacgagcg | 1900 |
1901 | tgacaccacg | atgcctgcag | caatggcaac | aacgttgcgc | aaactattaa | 1950 |
1951 | ctggcgaact | acttactcta | gcttcccggc | aacaattaat | agactggatg | 2000 |
2001 | gaggcggata | aagttgcagg | accacttctg | cgctcggccc | ttccggctgg | 2050 |
2051 | ctggtttatt | gctgataaat | ctggagccgg | tgagcgtggg | tctcgcggta | 2100 |
2101 | tcattgcagc | actggggcca | gatggtaagc | cctcccgtat | cgtagttatc | 2150 |
2151 | tacacgacgg | ggagtcaggc | aactatggat | gaacgaaata | gacagatcgc | 2200 |
2201 | tgagataggt | gcctcactga | ttaagcattg | gtaactgtca | gaccaagttt | 2250 |
2251 | actcatatat | actttagatt | gatttaaaac | ttcattttta | atttaaaagg | 2300 |
2301 | atctaggtga | agatcctttt | tgataatctc | atgaccaaaa | tcccttaacg | 2350 |
2351 | tgagttttcg | ttccactgag | cgtcagaccc | cgtagaaaag | atcaaaggat | 2400 |
2401 | cttcttgaga | tccttttttt | ctgcgcgtaa | tctgctgctt | gcaaacaaaa | 2450 |
2451 | aaaccaccgc | taccagcggt | ggtttgtttg | ccggatcaag | agctaccaac | 2500 |
2501 | tctttttccg | aaggtaactg | gcttcagcag | agcgcagata | ccaaatactg | 2550 |
2551 | tccttctagt | gtagccgtag | ttaggccacc | acttcaagaa | ctctgtagca | 2600 |
2601 | ccgcctacat | acctcgctct | gctaatcctg | ttaccagtgg | ctgctgccag | 2650 |
2651 | tggcgataag | tcgtgtctta | ccgggttgga | ctcaagacga | tagttaccgg | 2700 |
2701 | ataaggcgca | gcggtcgggc | tgaacggggg | gttcgtgcac | acagcccagc | 2750 |
2751 | ttggagcgaa | cgacctacac | cgaactgaga | tacctacagc | gtgagctatg | 2800 |
2801 | agaaagcgcc | acgcttcccg | aagggagaaa | ggcggacagg | tatccggtaa | 2850 |
2851 | gcggcagggt | cggaacagga | gagcgcacga | gggagcttcc | agggggaaac | 2900 |
2901 | gcctggtatc | tttatagtcc | tgtcgggttt | cgccacctct | gacttgagcg | 2950 |
2951 | tcgatttttg | tgatgctcgt | caggggggcg | gagcctatgg | aaaaacgcca | 3000 |
3001 | gcaacgcggc | ctttttacgg | ttcctggcct | tttgctggcc | ttttgctcac | 3050 |
3051 | atgttctttc | ctgcgttatc | ccctgattct | gtggataacc | gtattaccgc | 3100 |
3101 | ctttgagtga | gctgataccg | ctcgccgcag | ccgaacgacc | gagcgcagcg | 3150 |
3151 | agtcagtgag | cgaggaagcg | gaagagcgcc | tgatgcggta | ttttctcctt | 3200 |
3201 | acgcatctgt | gcggtatttc | acaccgcata | aattccgaca | ccatcgaatg | 3250 |
3251 | gtgcaaaacc | tttcgcggta | tggcatgata | gcgcccggaa | gagagtcaat | 3300 |
3301 | tcagggtggt | gaatgtgaaa | ccagtaacgt | tatacgatgt | cgcagagtat | 3350 |
3351 | gccggtgtct | cttatcagac | cgtttcccgc | gtggtgaacc | aggccagcca | 3400 |
3401 | cgtttctgcg | aaaacgcggg | aaaaagtgga | agcggcgatg | gcggagctga | 3450 |
3451 | attacattcc | caaccgcgtg | gcacaacaac | tggcgggcaa | acagtcgttg | 3500 |
3501 | ctgattggcg | ttgccacctc | cagtctggcc | ctgcacgcgc | cgtcgcaaat | 3550 |
3551 | tgtcgcggcg | attaaatctc | gcgccgatca | actgggtgcc | agcgtggtgg | 3600 |
3601 | tgtcgatggt | agaacgaagc | ggcgtcgaag | cctgtaaagc | ggcggtgcac | 3650 |
3651 | aatcttctcg | cgcaacgcgt | cagtgggctg | atcattaact | atccgctgga | 3700 |
3701 | tgaccaggat | gccattgctg | tggaagctgc | ctgcactaat | gttccggcgt | 3750 |
3751 | tatttcttga | tgtctctgac | cagacaccca | tcaacagtat | tattttctcc | 3800 |
3801 | catgaagacg | gtacgcgact | gggcgtggag | catctggtcg | cattgggtca | 3850 |
3851 | ccagcaaatc | gcgctgttag | cgggcccatt | aagttctgtc | tcggcgcgtc | 3900 |
3901 | tgcgtctggc | tggctggcat | aaatatctca | ctcgcaatca | aattcagccg | 3950 |
3951 | atagcggaac | gggaaggcga | ctggagtgcc | atgtccggtt | ttcaacaaac | 4000 |
4001 | catgcaaatg | ctgaatgagg | gcatcgttcc | cactgcgatg | ctggttgcca | 4050 |
4051 | acgatcagat | ggcgctgggc | gcaatgcgcg | ccattaccga | gtccgggctg | 4100 |
4101 | cgcgttggtg | cggatatctc | ggtagtggga | tacgacgata | ccgaagacag | 4150 |
4151 | ctcatgttat | atcccgccgt | taaccaccat | caaacaggat | tttcgcctgc | 4200 |
4201 | tggggcaaac | cagcgtggac | cgcttgctgc | aactctctca | gggccaggcg | 4250 |
4251 | gtgaagggca | atcagctgtt | gcccgtctca | ctggtgaaaa | gaaaaaccac | 4300 |
4301 | cctggcgccc | aatacgcaaa | ccgcctctcc | ccgcgcgttg | gccgattcat | 4350 |
4351 | taatgcagct | ggcacgacag | gtttcccgac | tggaaagcgg | gcagtgagcg | 4400 |
4401 | caacgcaatt | aatgtgagtt | agctcactca | ttaggcaccc | caggctttac | 4450 |
4451 | actttatgct | tccggctcgt | atgttgtgtg | gaattgtgag | cggataacaa | 4500 |
4501 | tttcacacag | gaaacagcta | tgaccatgat | tacggattca | ctggccgtcg | 4550 |
4551 | ttttacaacg | tcgtgactgg | gaaaaccctg | gcgttaccca | acttaatcgc | 4600 |
4601 | cttgcagcac | atcccccttt | cgccagctgg | cgtaatagcg | aagaggcccg | 4650 |
4651 | caccgatcgc | ccttcccaac | agttgcgcag | cctgaatggc | gaatggcgct | 4700 |
4701 | ttgcctggtt | tccggcacca | gaagcggtgc | cggaaagctg | gctggagtgc | 4750 |
4751 | gatcttcctg | aggccgatac | tgtcgtcgtc | ccctcaaact | ggcagatgca | 4800 |
4801 | cggttacgat | gcgcccatct | acaccaacgt | aacctatccc | attacggtca | 4850 |
4851 | atccgccgtt | tgttcccacg | gagaatccga | cgggttgtta | ctcgctcaca | 4900 |
4901 | tttaatgttg | atgaaagctg | gctacaggaa | ggccagacgc | gaattatttt | 4950 |
4951 | tgatggcgtt | ggaatt | 4966 |
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