手机验证
—20℃,12个月
2年
20
康朗生物
pmirGLO
5ug
质粒类型: | 双萤光素酶报告基因载体 |
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克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7350 bp (查看载体序列) |
载体抗性: | Ampicillin (氨苄青霉素) |
产品编号 | 产品名称 | 规格 | 价格 |
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KL1439 | pmirGLO | 5ug质粒 |
询价 |
The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a–d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3´ of the firefly luciferase gene (luc2 ). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3´ untranslated region (UTR) of a gene of interest, to study the influence of these sites on transcript stability and activity. Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2 ) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection. This vector contains the following features:
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