pFL59-

名称	编号	规格	价格	手册
pFL59-

质粒编号:
质粒名称: pFL59-
其他名称:
质粒大小（bp）: 4798
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X70271
其他属性: pFL59- was constructed from pGEM-3Zf(-) (Promega) and pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the yeast replication origin in the ClaI site. The 1.4 kb BglI fragment of the pGEM3Zf- plasmid bearing the 0.45 kb phage f1 region was used to replace the coresponding BglI fragment in pFL39. The order of the major features in this plasmid is:EcoRI/MCS/HindIII – TRP1 – ARS-CEN6 – pMB1 ori – ampR – f1 ori. YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. Restriction digests of the clone give the following sizes (kb): BamHI–4.8; BglII–4.0, 0.85; ClaI–4.0, 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397136 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid, pGEM-3Zf+.
质粒图谱:

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tgagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accatatgcg	gtgtgaaata	200
201	ccgcacagat	gcgtaaggag	aaaataccgc	atcaggccgc	gccctgtagc	250
251	ggcgcattaa	gcgcggcggg	tgtggtggtt	acgcgcagcg	tgaccgctac	300
301	acttgccagc	gccctagcgc	ccgctccttt	cgctttcttc	ccttcctttc	350
351	tcgccacgtt	cgccggcttt	ccccgtcaag	ctctaaatcg	ggggctccct	400
401	ttagggttcc	gatttagtgc	tttacggcac	ctcgacccca	aaaaacttga	450
451	ttagggtgat	ggttcacgta	gtgggccatc	gccctgatag	acggtttttc	500
501	gccctttgac	gttggagtcc	acgttcttta	atagtggact	cttgttccaa	550
551	actggaacaa	cactcaaccc	tatctcggtc	tattcttttg	atttataagg	600
601	gattttgccg	atttcggcct	attggttaaa	atgagctgat	ttaacaaaaa	650
651	tttaacgcga	attttaacaa	aatattaacg	tgccattcgc	cattcaggct	700
701	gcgcaactgt	tgggaagggc	gatcggtgcg	ggcctcttcg	ctattacgcc	750
751	agctggcgaa	agggggatgt	gctgcaaggc	gattaagttg	ggtaacgcca	800
801	gggttttccc	agtcacgacg	ttgtaaaacg	acggccagtg	aattcgagct	850
851	cggtacccgg	ggatcctcta	gagtcgacct	gcaggcatgc	aagcttggcg	900
901	taatcatggt	catagctgtt	tcctgtgtga	aattgttatc	cgctcacaat	950
951	tccacacaac	atacgagccg	gaagcataaa	gtgtaaagcc	tggggtgcct	1000
1001	aatgagtgag	ctaactcaca	ttaattgcgt	tgcgctcact	gcccgctttc	1050
1051	cagtcgggaa	acctgtcgtg	ccagcagatc	tgggcaagtg	cacaaacaat	1100
1101	acttaaataa	atactactca	gtaataacct	atttcttagc	atttttgacg	1150
1151	aaatttgcta	ttttgttaga	gtcttttaca	ccatttgtct	ccacacctcc	1200
1201	gcttacatca	acaccaataa	cgccatttaa	tctaagcgca	tcaccaacat	1250
1251	tttctggcgt	cagtccacca	gctaacataa	aatgtaaact	ttcggggctc	1300
1301	tcttgccttc	caacccagtc	agaaatcgag	ttccaatcca	aaagttcacc	1350
1351	tgtcccacct	gcttctgaat	caaacaaggg	aataaacgaa	tgaggtttct	1400
1401	gtgaagctgc	actgagtagt	atgttgcagt	cttttggaaa	tacgagtctt	1450
1451	ttaataactg	gcaaaccgag	gaactcttgg	tattcttgcc	acgactcatc	1500
1501	tccatgcagt	tggacgatat	caatgccgta	atcattgacc	agagccaaaa	1550
1551	catcctcctt	aggttgatta	cgaaacacgc	caaccaagta	tttcggagtg	1600
1601	cctgaactat	ttttatatgc	ttttacaaga	cttgaaattt	tccttgcaat	1650
1651	aaccgggtca	attgttctct	ttctattggg	cacacatata	atacccagca	1700
1701	agtcagcatc	ggaatctaga	gcacattctg	cggcctctgt	gctctgcaag	1750
1751	ccgcaaactt	tcaccaatgg	accagaacta	cctgtgaaat	taataacaga	1800
1801	catactccaa	gctgcctttg	tgtgcttaat	cacggtatat	atactcacgt	1850
1851	gctcaatagt	caccaatgcc	ctccctcttg	gccctctcct	tttctttttt	1900
1901	cgaccgaatt	aattcagatc	tgctgcatta	atgaatcggc	caacgcgcgg	1950
1951	ggagaggcgg	tttgcgtatt	gggcgctctt	ccgcttcctc	gctcactgac	2000
2001	tcgctgcgct	cggtcgttcg	gctgcggcga	gcggtatcag	catcgatgaa	2050
2051	tggaaattac	gtactttcaa	cgcaagagca	agacaaaaga	aagcctaaaa	2100
2101	ctgtcttttc	gtgtcggtcg	tccaatatca	tcgtaaacgt	gtgtagagcc	2150
2151	ttaaattcta	atttctaact	ttaaattttt	tttgctatta	taatactaat	2200
2201	ttctaacttt	caacctattt	tacatcttcg	gaaaacaaaa	actatttttt	2250
2251	ctttaatttc	tttttttact	ttctattttt	aatttatata	tttatattaa	2300
2301	aaaatttaaa	ttatattatt	tttatagcac	gtgatgaaaa	gaaatatagt	2350
2351	cttcaaggtt	atattataca	ggtttatata	ttattttact	tatttttgtt	2400
2401	gttctttttc	ttggagtaat	ttagcattaa	caacttcgac	ccatcgcgat	2450
2451	gacgaaattg	cacgtttaaa	ggagaagtcg	gaatcgttgc	aagaagaaat	2500
2501	aaattagttt	gacgttttat	tccttttctt	ctgcacagaa	gtgtgtatat	2550
2551	atatatttat	atatagtcga	tgtgcaaaag	cctacattat	ttttcttttg	2600
2601	atttttattt	ttttgttcaa	gtaggttcat	gccgacagta	aatccttatg	2650
2651	ccctacctaa	atataaacac	ccaaatatat	atatatatat	atatatatat	2700
2701	aaataataaa	aacggaaagg	aaatagaata	ataataataa	tactacacgt	2750
2751	ttacgtatgt	aactcattct	attattcaga	ctttgatatt	aatagaatta	2800
2801	ttactactag	gtttcctcac	ttcaaaatct	ataaatatac	cgtcatgctc	2850
2851	taatcgatgc	tcactcaaag	gtcggtaata	cggttatcca	cagaatcagg	2900
2901	ggataacgca	ggaaagaaca	tgtgagcaaa	aggccagcaa	aaggccagga	2950
2951	accgtaaaaa	ggccgcgttg	ctggcgtttt	tccataggct	ccgcccccct	3000
3001	gacgagcatc	acaaaaatcg	acgctcaagt	cagaggtggc	gaaacccgac	3050
3051	aggactataa	agataccagg	cgtttccccc	tggaagctcc	ctcgtgcgct	3100
3101	ctcctgttcc	gaccctgccg	cttaccggat	acctgtccgc	ctttctccct	3150
3151	tcgggaagcg	tggcgctttc	tcatagctca	cgctgtaggt	atctcagttc	3200
3201	ggtgtaggtc	gttcgctcca	agctgggctg	tgtgcacgaa	ccccccgttc	3250
3251	agcccgaccg	ctgcgcctta	tccggtaact	atcgtcttga	gtccaacccg	3300
3301	gtaagacacg	acttatcgcc	actggcagca	gccactggta	acaggattag	3350
3351	cagagcgagg	tatgtaggcg	gtgctacaga	gttcttgaag	tggtggccta	3400
3401	actacggcta	cactagaagg	acagtatttg	gtatctgcgc	tctgctgaag	3450
3451	ccagttacct	tcggaaaaag	agttggtagc	tcttgatccg	gcaaacaaac	3500
3501	caccgctggt	agcggtggtt	tttttgtttg	caagcagcag	attacgcgca	3550
3551	gaaaaaaagg	atctcaagaa	gatcctttga	tcttttctac	ggggtctgac	3600
3601	gctcagtgga	acgaaaactc	acgttaaggg	attttggtca	tgagattatc	3650
3651	aaaaaggatc	ttcacctaga	tccttttaaa	ttaaaaatga	agttttaaat	3700
3701	caatctaaag	tatatatgag	taaacttggt	ctgacagtta	ccaatgctta	3750
3751	atcagtgagg	cacctatctc	agcgatctgt	ctatttcgtt	catccatagt	3800
3801	tgcctgactc	cccgtcgtgt	agataactac	gatacgggag	ggcttaccat	3850
3851	ctggccccag	tgctgcaatg	ataccgcgag	acccacgctc	accggctcca	3900
3901	gatttatcag	caataaacca	gccagccgga	agggccgagc	gcagaagtgg	3950
3951	tcctgcaact	ttatccgcct	ccatccagtc	tattaattgt	tgccgggaag	4000
4001	ctagagtaag	tagttcgcca	gttaatagtt	tgcgcaacgt	tgttggcatt	4050
4051	gctacaggca	tcgtggtgtc	acgctcgtcg	tttggtatgg	cttcattcag	4100
4101	ctccggttcc	caacgatcaa	ggcgagttac	atgatccccc	atgttgtgca	4150
4151	aaaaagcggt	tagctccttc	ggtcctccga	tcgttgtcag	aagtaagttg	4200
4201	gccgcagtgt	tatcactcat	ggttatggca	gcactgcata	attctcttac	4250
4251	tgtcatgcca	tcggtaagat	gcttttctgt	gactggtgag	tactcaacca	4300
4301	agtcattctg	agaatagtgt	atgcggcgac	cgagttgctc	ttgcccggcg	4350
4351	tcaatacggg	ataatagtgt	atgacatagc	agaactttaa	aagtgctcat	4400
4401	cattggaaaa	cgttcttcgg	ggcgaaaact	ctcaaggatc	ttaccgctgt	4450
4451	tgagatccag	ttcgatgtaa	cccactcgtg	cacccaactg	atcttcagca	4500
4501	tcttttactt	tcaccagcgt	ttctgggtga	gcaaaaacag	gaaggcaaaa	4550
4551	tgccgcaaaa	aagggaataa	gggcgacacg	gaaatgttga	atactcatac	4600
4601	tcttcctttt	tcaatattat	tgaagcattt	atcagggtta	ttgtctcatg	4650
4651	agcggataca	tatttgaatg	tatttagaaa	aataaacaaa	taggggttcc	4700
4701	gcgcacattt	ccccgaaaag	tgccacctga	cgtctaagaa	accattatta	4750
4751	tcatgacatt	aacctataaa	aataggcgta	tcacgaggcc	ctttcgtc	4798