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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
sion High-Fidelity DNA Polymerase (2 U/µL)
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
100 units
描述
Thermo Scientific Phusion高保真度DNA聚合酶设定了PCR性能的金标准。本品提供2 U/µL、100 Units的包装形式,Phusion高保真DNA聚合酶的错配率比Taq酶低50倍,比优化条件下的Pfu酶低6倍。Phusion高保真度DNA聚合酶是目前最为精确的热稳定性聚合酶,是分子克隆及其他需要高保真性操作的最佳选择。该酶的独特结构决定其具有较短的反应时间,很高的得率和强大的性能,甚至能胜任PCR抑制剂存在的反应条件。对于研究人员来说,这代表了易用性和便捷性。此外,Phusion DNA聚合酶相比其他DNA聚合酶而言,能够在酶量更少的条件下获得更高的得率。• Phusion DNA聚合酶2 U/µL
• 5X Phusion HF缓冲液
• 5X Phusion GC缓冲液
• DMSO
• 50 mM MgCl2 溶液
Phusion HF缓冲液和Phusion GC缓冲液在1X浓度条件下均含有1.5 mM MgCl2。
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文献和实验Expand High Fidelity PCR--高保真PCR
Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
. DNA can be quantified and diluted to a working concentration at this point or simply use 1 µl per PCR reaction. We expect that the yield of this procedure be 100 to 300 ng/µl, DNA. Using the above method, high quality DNA samples from a sheep
SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
. Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum® Taq DNA Polymerase High Fidelity is an enzyme mixture composed of recombinant Taq DNA polymerase
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