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低温运输,-20℃保存
1年
pET-42c(+)
大量
信裕生物
名称 | 编号 | 规格 | 价格 | 手册 |
---|---|---|---|---|
pET-42c(+)(pET42c) |
质粒编号:
质粒名称: pET-42c(+)
其他名称:
质粒大小(bp): 5931
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa
5′ 测序引物: T7或pGEX-5′
5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′; pGEX-5′: 5′-GGGCTGGCAAGCCACGTTTGGTG-3′
GenBank号:
其他属性: Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm Factor Xa cleavage site; a,b,c vary by MCS
质粒图谱:
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 120 | 1 | |
T7_Terminal_primer | 69 | 87 | |
S15 | 352 | 308 | |
6xHIS | 412 | 395 | |
GST (variant) | 1093 | 443 | |
T7_transl_en_RBS | 1118 | 1102 | |
lacO | 1163 | 1136 | |
T7_promoter | 1181 | 1163 | |
tet (300 – 563) | 1217 | 1480 | |
pBRrevBam_primer | 1288 | 1269 | |
lacI | 1563 | 2654 | |
ROP | 3226 | 3417 | |
pGEX_3_primer | 3433 | 3411 | |
pBR322_origin | 4451 | 3832 | |
KanR2 | 4557 | 5372 |
ORF | Start | End | |
---|---|---|---|
ORF frame 3 | 1093 | 143 | |
ORF frame 2 | 1094 | 1822 | |
ORF frame 3 | 1695 | 2654 | |
ORF frame 3 | 4557 | 5372 |
Enzyme Name | Cut |
---|---|
XhoI | 174 |
NotI | 182 |
EagI | 182 |
HindIII | 189 |
SalI | 195 |
SacI | 206 |
PstI | 214 |
AscI | 216 |
StuI | 226 |
EcoRI | 235 |
BamHI | 241 |
EcoRV | 251 |
NcoI | 256 |
AgeI | 290 |
KpnI | 297 |
BglII | 300 |
SacII | 391 |
SpeI | 422 |
MscI | 885 |
NdeI | 1092 |
XbaI | 1130 |
ApaI | 2133 |
HpaI | 2428 |
NruI | 4645 |
SmaI | 4862 |
XmaI | 4860 |
质粒序列:
1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggtatt | aatcaattag | 150 |
151 | tggtggtggt | ggtggtggtg | gtgctcgagt | gcggccgcaa | gcttgtcgac | 200 |
201 | ggagctcgcc | tgcaggcgcg | ccaaggcctg | tacagaattc | ggatccccga | 250 |
251 | tatcgccatg | gacccgcgtc | cctcaatacc | ggagccacca | ccggtaccca | 300 |
301 | gatctgggct | gtccatgtgc | tggcgttcga | atttagcagc | agcggtttct | 350 |
351 | ttcataccaa | ttgcagtact | accgcgtggc | accagacccg | cggagtgatg | 400 |
401 | gtgatggtga | tgaccagaac | cactagttga | accatccgat | tttggaggat | 450 |
451 | ggtcgccacc | accaaacgtg | gcttgccagc | cctgcaaagg | ccatgctata | 500 |
501 | tacttgctgg | atttcaagta | cttatcaatt | tgtgggatag | cttcaatacg | 550 |
551 | ttttttaaaa | caaactaatt | ttgggaacgc | atccaggcac | attgggtcca | 600 |
601 | tgtataaaac | aacatcaaga | gcgtcataca | acatgaagtc | aggatgggtt | 650 |
651 | acatgatcac | catttaaata | tgttttatga | cataaacgat | cttcgaacat | 700 |
701 | tttcagcatt | tcaggtagct | tgctaagaaa | atcaactttg | agagtttcaa | 750 |
751 | agtctttact | atatgcaatt | ctcgaaacac | cgtatctaat | atccaaaacc | 800 |
801 | gctccttcaa | gcattgaaat | ctctgcacgc | tcttttggac | aaccacccaa | 850 |
851 | catgttgtgc | ttgtcagcta | tataacgtat | gatggccata | gactgtgtta | 900 |
901 | atttaacatc | accatcaata | taataaggaa | gattgggaaa | ctccaaaccc | 950 |
951 | aattcaaact | ttttgtttcg | ccatttatca | ccttcatcgc | gctcatacaa | 1000 |
1001 | atgctcttca | tatttttctt | caagatattc | caaaagaagt | cgagtgggtt | 1050 |
1051 | gcacaaggcc | cttaattttc | caataaccta | gtatagggga | catatgtata | 1100 |
1101 | tctccttctt | aaagttaaac | aaaattattt | ctagagggga | attgttatcc | 1150 |
1151 | gctcacaatt | cccctatagt | gagtcgtatt | aatttcgcgg | gatcgagatc | 1200 |
1201 | gatctcgatc | ctctacgccg | gacgcatcgt | ggccggcatc | accggcgcca | 1250 |
1251 | caggtgcggt | tgctggcgcc | tatatcgccg | acatcaccga | tggggaagat | 1300 |
1301 | cgggctcgcc | acttcgggct | catgagcgct | tgtttcggcg | tgggtatggt | 1350 |
1351 | ggcaggcccc | gtggccgggg | gactgttggg | cgccatctcc | ttgcatgcac | 1400 |
1401 | cattccttgc | ggcggcggtg | ctcaacggcc | tcaacctact | actgggctgc | 1450 |
1451 | ttcctaatgc | aggagtcgca | taagggagag | cgtcgagatc | ccggacacca | 1500 |
1501 | tcgaatggcg | caaaaccttt | cgcggtatgg | catgatagcg | cccggaagag | 1550 |
1551 | agtcaattca | gggtggtgaa | tgtgaaacca | gtaacgttat | acgatgtcgc | 1600 |
1601 | agagtatgcc | ggtgtctctt | atcagaccgt | ttcccgcgtg | gtgaaccagg | 1650 |
1651 | ccagccacgt | ttctgcgaaa | acgcgggaaa | aagtggaagc | ggcgatggcg | 1700 |
1701 | gagctgaatt | acattcccaa | ccgcgtggca | caacaactgg | cgggcaaaca | 1750 |
1751 | gtcgttgctg | attggcgttg | ccacctccag | tctggccctg | cacgcgccgt | 1800 |
1801 | cgcaaattgt | cgcggcgatt | aaatctcgcg | ccgatcaact | gggtgccagc | 1850 |
1851 | gtggtggtgt | cgatggtaga | acgaagcggc | gtcgaagcct | gtaaagcggc | 1900 |
1901 | ggtgcacaat | cttctcgcgc | aacgcgtcag | tgggctgatc | attaactatc | 1950 |
1951 | cgctggatga | ccaggatgcc | attgctgtgg | aagctgcctg | cactaatgtt | 2000 |
2001 | ccggcgttat | ttcttgatgt | ctctgaccag | acacccatca | acagtattat | 2050 |
2051 | tttctcccat | gaagacggta | cgcgactggg | cgtggagcat | ctggtcgcat | 2100 |
2101 | tgggtcacca | gcaaatcgcg | ctgttagcgg | gcccattaag | ttctgtctcg | 2150 |
2151 | gcgcgtctgc | gtctggctgg | ctggcataaa | tatctcactc | gcaatcaaat | 2200 |
2201 | tcagccgata | gcggaacggg | aaggcgactg | gagtgccatg | tccggttttc | 2250 |
2251 | aacaaaccat | gcaaatgctg | aatgagggca | tcgttcccac | tgcgatgctg | 2300 |
2301 | gttgccaacg | atcagatggc | gctgggcgca | atgcgcgcca | ttaccgagtc | 2350 |
2351 | cgggctgcgc | gttggtgcgg | acatctcggt | agtgggatac | gacgataccg | 2400 |
2401 | aagacagctc | atgttatatc | ccgccgttaa | ccaccatcaa | acaggatttt | 2450 |
2451 | cgcctgctgg | ggcaaaccag | cgtggaccgc | ttgctgcaac | tctctcaggg | 2500 |
2501 | ccaggcggtg | aagggcaatc | agctgttgcc | cgtctcactg | gtgaaaagaa | 2550 |
2551 | aaaccaccct | ggcgcccaat | acgcaaaccg | cctctccccg | cgcgttggcc | 2600 |
2601 | gattcattaa | tgcagctggc | acgacaggtt | tcccgactgg | aaagcgggca | 2650 |
2651 | gtgagcgcaa | cgcaattaat | gtaagttagc | tcactcatta | ggcaccggga | 2700 |
2701 | tctcgaccga | tgcccttgag | agccttcaac | ccagtcagct | ccttccggtg | 2750 |
2751 | ggcgcggggc | atgactagca | tgatcgtgct | cctgtcgttg | aggacccggc | 2800 |
2801 | taggctggcg | gggttgcctt | actggttagc | agaatgaatc | accgatacgc | 2850 |
2851 | gagcgaacgt | gaagcgactg | ctgctgcaaa | acgtctgcga | cctgagcaac | 2900 |
2901 | aacatgaatg | gtcttcggtt | tccgtgtttc | gtaaagtctg | gaaacgcgga | 2950 |
2951 | agtcagcgcc | ctgcaccatt | atgttccgga | tctgcatcgc | aggatgctgc | 3000 |
3001 | tggctaccct | gtggaacacc | tacatctgta | ttaacgaagc | gctggcattg | 3050 |
3051 | accctgagtg | atttttctct | ggtcccgccg | catccatacc | gccagttgtt | 3100 |
3101 | taccctcaca | acgttccagt | aaccgggcat | gttcatcatc | agtaacccgt | 3150 |
3151 | atcgtgagca | tcctctctcg | tttcatcggt | atcattaccc | ccatgaacag | 3200 |
3201 | aaatccccct | tacacggagg | catcagtgac | caaacaggaa | aaaaccgccc | 3250 |
3251 | ttaacatggc | ccgctttatc | agaagccaga | cattaacgct | tctggagaaa | 3300 |
3301 | ctcaacgagc | tggacgcgga | tgaacaggca | gacatctgtg | aatcgcttca | 3350 |
3351 | cgaccacgct | gatgagcttt | accgcagctg | cctcgcgcgt | ttcggtgatg | 3400 |
3401 | acggtgaaaa | cctctgacac | atgcagctcc | cggagacggt | cacagcttgt | 3450 |
3451 | ctgtaagcgg | atgccgggag | cagacaagcc | cgtcagggcg | cgtcagcggg | 3500 |
3501 | tgttggcggg | tgtcggggcg | cagccatgac | ccagtcacgt | agcgatagcg | 3550 |
3551 | gagtgtatac | tggcttaact | atgcggcatc | agagcagatt | gtactgagag | 3600 |
3601 | tgcaccatat | atgcggtgtg | aaataccgca | cagatgcgta | aggagaaaat | 3650 |
3651 | accgcatcag | gcgctcttcc | gcttcctcgc | tcactgactc | gctgcgctcg | 3700 |
3701 | gtcgttcggc | tgcggcgagc | ggtatcagct | cactcaaagg | cggtaatacg | 3750 |
3751 | gttatccaca | gaatcagggg | ataacgcagg | aaagaacatg | tgagcaaaag | 3800 |
3801 | gccagcaaaa | ggccaggaac | cgtaaaaagg | ccgcgttgct | ggcgtttttc | 3850 |
3851 | cataggctcc | gcccccctga | cgagcatcac | aaaaatcgac | gctcaagtca | 3900 |
3901 | gaggtggcga | aacccgacag | gactataaag | ataccaggcg | tttccccctg | 3950 |
3951 | gaagctccct | cgtgcgctct | cctgttccga | ccctgccgct | taccggatac | 4000 |
4001 | ctgtccgcct | ttctcccttc | gggaagcgtg | gcgctttctc | atagctcacg | 4050 |
4051 | ctgtaggtat | ctcagttcgg | tgtaggtcgt | tcgctccaag | ctgggctgtg | 4100 |
4101 | tgcacgaacc | ccccgttcag | cccgaccgct | gcgccttatc | cggtaactat | 4150 |
4151 | cgtcttgagt | ccaacccggt | aagacacgac | ttatcgccac | tggcagcagc | 4200 |
4201 | cactggtaac | aggattagca | gagcgaggta | tgtaggcggt | gctacagagt | 4250 |
4251 | tcttgaagtg | gtggcctaac | tacggctaca | ctagaaggac | agtatttggt | 4300 |
4301 | atctgcgctc | tgctgaagcc | agttaccttc | ggaaaaagag | ttggtagctc | 4350 |
4351 | ttgatccggc | aaacaaacca | ccgctggtag | cggtggtttt | tttgtttgca | 4400 |
4401 | agcagcagat | tacgcgcaga | aaaaaaggat | ctcaagaaga | tcctttgatc | 4450 |
4451 | ttttctacgg | ggtctgacgc | tcagtggaac | gaaaactcac | gttaagggat | 4500 |
4501 | tttggtcatg | aacaataaaa | ctgtctgctt | acataaacag | taatacaagg | 4550 |
4551 | ggtgttatga | gccatattca | acgggaaacg | tcttgctcta | ggccgcgatt | 4600 |
4601 | aaattccaac | atggatgctg | atttatatgg | gtataaatgg | gctcgcgata | 4650 |
4651 | atgtcgggca | atcaggtgcg | acaatctatc | gattgtatgg | gaagcccgat | 4700 |
4701 | gcgccagagt | tgtttctgaa | acatggcaaa | ggtagcgttg | ccaatgatgt | 4750 |
4751 | tacagatgag | atggtcagac | taaactggct | gacggaattt | atgcctcttc | 4800 |
4801 | cgaccatcaa | gcattttatc | cgtactcctg | atgatgcatg | gttactcacc | 4850 |
4851 | actgcgatcc | ccgggaaaac | agcattccag | gtattagaag | aatatcctga | 4900 |
4901 | ttcaggtgaa | aatattgttg | atgcgctggc | agtgttcctg | cgccggttgc | 4950 |
4951 | attcgattcc | tgtttgtaat | tgtcctttta | acagcgatcg | cgtatttcgt | 5000 |
5001 | ctcgctcagg | cgcaatcacg | aatgaataac | ggtttggttg | atgcgagtga | 5050 |
5051 | ttttgatgac | gagcgtaatg | gctggcctgt | tgaacaagtc | tggaaagaaa | 5100 |
5101 | tgcataaact | tttgccattc | tcaccggatt | cagtcgtcac | tcatggtgat | 5150 |
5151 | ttctcacttg | ataaccttat | ttttgacgag | gggaaattaa | taggttgtat | 5200 |
5201 | tgatgttgga | cgagtcggaa | tcgcagaccg | ataccaggat | cttgccatcc | 5250 |
5251 | tatggaactg | cctcggtgag | ttttctcctt | cattacagaa | acggcttttt | 5300 |
5301 | caaaaatatg | gtattgataa | tcctgatatg | aataaattgc | agtttcattt | 5350 |
5351 | gatgctcgat | gagtttttct | aagaattaat | tcatgagcgg | atacatattt | 5400 |
5401 | gaatgtattt | agaaaaataa | acaaataggg | gttccgcgca | catttccccg | 5450 |
5451 | aaaagtgcca | cctgaaattg | taaacgttaa | tattttgtta | aaattcgcgt | 5500 |
5501 | taaatttttg | ttaaatcagc | tcatttttta | accaataggc | cgaaatcggc | 5550 |
5551 | aaaatccctt | ataaatcaaa | agaatagacc | gagatagggt | tgagtgttgt | 5600 |
5601 | tccagtttgg | aacaagagtc | cactattaaa | gaacgtggac | tccaacgtca | 5650 |
5651 | aagggcgaaa | aaccgtctat | cagggcgatg | gcccactacg | tgaaccatca | 5700 |
5701 | ccctaatcaa | gttttttggg | gtcgaggtgc | cgtaaagcac | taaatcggaa | 5750 |
5751 | ccctaaaggg | agcccccgat | ttagagcttg | acggggaaag | ccggcgaacg | 5800 |
5801 | tggcgagaaa | ggaagggaag | aaagcgaaag | gagcgggcgc | tagggcgctg | 5850 |
5851 | gcaagtgtag | cggtcacgct | gcgcgtaacc | accacacccg | ccgcgcttaa | 5900 |
5901 | tgcgccgcta | cagggcgcgt | cccattcgcc | a | 5931 |
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