pET-39b(+)

名称	编号	规格	价格	手册
pET-39b(+)(pET39b)(60908-2804)y	60908-2804y

质粒编号: 60908-2804y
质粒名称: pET-39b(+)
其他名称: pET39b, pet 39b
质粒大小（bp）: 6106
抗性: Kanamycin (卡那霉素)
质粒类型:大肠杆菌(Escherichia coli)蛋白表达
是否病毒: 非病毒
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: His (中间和C端), N-DsbA, N-S, N-EK, N-Thrombin
5′ 测序引物及序列:T7: 5′-TAATACGACTCACTATAGGG-3′

3’测序引物及序列：无
GenBank号:
其他属性:Encodes Dsb tag for export and periplasmic folding of target proteins

质粒图谱:

Feature Name	Start	End
T7_terminator	120	1
T7_Terminal_primer	69	87
EK	266	252
S15	326	282
6xHIS	386	369
T7_transl_en_RBS	1056	1040
lacO	1101	1074
T7_promoter	1119	1101
tet (300 – 563)	1155	1418
pBRrevBam_primer	1226	1207
lacI	1501	2592
tet (576 – 851)	2651	2926
ROP	3401	3592
pGEX_3_primer	3608	3586
pBR322_origin	4626	4007
KanR2	4732	5547

ORF	Start	End
ORF frame 3	1031	147
ORF frame 3	1032	1760
ORF frame 1	1633	2592
ORF frame 1	4732	5547

Enzyme Name	Cut
XhoI	174
NotI	182
EagI	182
HindIII	189
SalI	195
SacI	206
EcoRI	208
BamHI	214
NcoI	227
KpnI	271
BstBI	301
SacII	365
SpeI	396
AflII	418
PstI	552
AscI	917
AgeI	928
NdeI	1030
XbaI	1068
BclI	1874
ApaI	2071
FspI	2942
NruI	4820

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgtttagcag	cctaggtatt	aatcaattag	150
151	tggtggtggt	ggtggtggtg	gtgctcgagt	gcggccgcaa	gcttgtcgac	200
201	ggagctcgaa	ttcggatccg	atatcgccat	ggttgaggag	aagcccgggc	250
251	tcttgtcgtc	gtcatcggta	cccagatctg	ggctgtccat	gtgctggcgt	300
301	tcgaatttag	cagcagcggt	ttctttcata	ccaattgcag	tactaccgcg	350
351	tggcaccaga	cccgcggagt	gatggtgatg	gtgatgacca	gaaccactag	400
401	ttgatccttt	tttctcgctt	aagtatttca	ctgtatcagc	atactgctga	450
451	acaaaaacat	ccatattgct	ggtatccata	ccctgcggat	tcagctgata	500
501	tttaccgtta	acaaacatcg	ccggaacgcc	acgcaattgc	acgtcagctg	550
551	cagctttttc	ctgctgagcg	accagagatt	tcaccacgaa	gctgttccac	600
601	gccgcgtcgt	actcttcacc	tttaatacct	gcgttgataa	atacatcgcg	650
651	gatatcagaa	gcagaacgaa	tggtctgggt	tttctgtacg	ccttcaaaca	700
701	gcggaacagt	cactttgtct	tccacgccca	gcgccatcgc	cacagcccat	750
751	gcctgagtca	gatctttgcc	caggtcacca	cccatgaagt	tgacgtggta	800
801	tttagtcatc	ttcacgcctt	ccggcagttt	tttcttcaca	ttatcagaaa	850
851	tatgcagaac	ttcttcaaac	tgatagcagt	gcgggcagaa	gaaagagaaa	900
901	aactccagca	cttgcggcgc	gccagctacc	ggtttttcca	gggtagtgta	950
951	ctgtttacca	tcttcatact	gcgccgccga	tgcgctaaac	gctaaaacta	1000
1001	aaccagccag	cgccagccaa	atctttttca	tatgtatatc	tccttcttaa	1050
1051	agttaaacaa	aattatttct	agaggggaat	tgttatccgc	tcacaattcc	1100
1101	cctatagtga	gtcgtattaa	tttcgcggga	tcgagatcga	tctcgatcct	1150
1151	ctacgccgga	cgcatcgtgg	ccggcatcac	cggcgccaca	ggtgcggttg	1200
1201	ctggcgccta	tatcgccgac	atcaccgatg	gggaagatcg	ggctcgccac	1250
1251	ttcgggctca	tgagcgcttg	tttcggcgtg	ggtatggtgg	caggccccgt	1300
1301	ggccggggga	ctgttgggcg	ccatctcctt	gcatgcacca	ttccttgcgg	1350
1351	cggcggtgct	caacggcctc	aacctactac	tgggctgctt	cctaatgcag	1400
1401	gagtcgcata	agggagagcg	tcgagatccc	ggacaccatc	gaatggcgca	1450
1451	aaacctttcg	cggtatggca	tgatagcgcc	cggaagagag	tcaattcagg	1500
1501	gtggtgaatg	tgaaaccagt	aacgttatac	gatgtcgcag	agtatgccgg	1550
1551	tgtctcttat	cagaccgttt	cccgcgtggt	gaaccaggcc	agccacgttt	1600
1601	ctgcgaaaac	gcgggaaaaa	gtggaagcgg	cgatggcgga	gctgaattac	1650
1651	attcccaacc	gcgtggcaca	acaactggcg	ggcaaacagt	cgttgctgat	1700
1701	tggcgttgcc	acctccagtc	tggccctgca	cgcgccgtcg	caaattgtcg	1750
1751	cggcgattaa	atctcgcgcc	gatcaactgg	gtgccagcgt	ggtggtgtcg	1800
1801	atggtagaac	gaagcggcgt	cgaagcctgt	aaagcggcgg	tgcacaatct	1850
1851	tctcgcgcaa	cgcgtcagtg	ggctgatcat	taactatccg	ctggatgacc	1900
1901	aggatgccat	tgctgtggaa	gctgcctgca	ctaatgttcc	ggcgttattt	1950
1951	cttgatgtct	ctgaccagac	acccatcaac	agtattattt	tctcccatga	2000
2001	agacggtacg	cgactgggcg	tggagcatct	ggtcgcattg	ggtcaccagc	2050
2051	aaatcgcgct	gttagcgggc	ccattaagtt	ctgtctcggc	gcgtctgcgt	2100
2101	ctggctggct	ggcataaata	tctcactcgc	aatcaaattc	agccgatagc	2150
2151	ggaacgggaa	ggcgactgga	gtgccatgtc	cggttttcaa	caaaccatgc	2200
2201	aaatgctgaa	tgagggcatc	gttcccactg	cgatgctggt	tgccaacgat	2250
2251	cagatggcgc	tgggcgcaat	gcgcgccatt	accgagtccg	ggctgcgcgt	2300
2301	tggtgcggac	atctcggtag	tgggatacga	cgataccgaa	gacagctcat	2350
2351	gttatatccc	gccgttaacc	accatcaaac	aggattttcg	cctgctgggg	2400
2401	caaaccagcg	tggaccgctt	gctgcaactc	tctcagggcc	aggcggtgaa	2450
2451	gggcaatcag	ctgttgcccg	tctcactggt	gaaaagaaaa	accaccctgg	2500
2501	cgcccaatac	gcaaaccgcc	tctccccgcg	cgttggccga	ttcattaatg	2550
2551	cagctggcac	gacaggtttc	ccgactggaa	agcgggcagt	gagcgcaacg	2600
2601	caattaatgt	aagttagctc	actcattagg	caccgggatc	tcgaccgatg	2650
2651	cccttgagag	ccttcaaccc	agtcagctcc	ttccggtggg	cgcggggcat	2700
2701	gactatcgtc	gccgcactta	tgactgtctt	ctttatcatg	caactcgtag	2750
2751	gacaggtgcc	ggcagcgctc	tgggtcattt	tcggcgagga	ccgctttcgc	2800
2801	tggagcgcga	cgatgatcgg	cctgtcgctt	gcggtattcg	gaatcttgca	2850
2851	cgccctcgct	caagccttcg	tcactggtcc	cgccaccaaa	cgtttcggcg	2900
2901	agaagcaggc	cattatcgcc	ggcatggcgg	ccccacgggt	gcgcatgatc	2950
2951	gtgctcctgt	cgttgaggac	ccggctaggc	tggcggggtt	gccttactgg	3000
3001	ttagcagaat	gaatcaccga	tacgcgagcg	aacgtgaagc	gactgctgct	3050
3051	gcaaaacgtc	tgcgacctga	gcaacaacat	gaatggtctt	cggtttccgt	3100
3101	gtttcgtaaa	gtctggaaac	gcggaagtca	gcgccctgca	ccattatgtt	3150
3151	ccggatctgc	atcgcaggat	gctgctggct	accctgtgga	acacctacat	3200
3201	ctgtattaac	gaagcgctgg	cattgaccct	gagtgatttt	tctctggtcc	3250
3251	cgccgcatcc	ataccgccag	ttgtttaccc	tcacaacgtt	ccagtaaccg	3300
3301	ggcatgttca	tcatcagtaa	cccgtatcgt	gagcatcctc	tctcgtttca	3350
3351	tcggtatcat	tacccccatg	aacagaaatc	ccccttacac	ggaggcatca	3400
3401	gtgaccaaac	aggaaaaaac	cgcccttaac	atggcccgct	ttatcagaag	3450
3451	ccagacatta	acgcttctgg	agaaactcaa	cgagctggac	gcggatgaac	3500
3501	aggcagacat	ctgtgaatcg	cttcacgacc	acgctgatga	gctttaccgc	3550
3551	agctgcctcg	cgcgtttcgg	tgatgacggt	gaaaacctct	gacacatgca	3600
3601	gctcccggag	acggtcacag	cttgtctgta	agcggatgcc	gggagcagac	3650
3651	aagcccgtca	gggcgcgtca	gcgggtgttg	gcgggtgtcg	gggcgcagcc	3700
3701	atgacccagt	cacgtagcga	tagcggagtg	tatactggct	taactatgcg	3750
3751	gcatcagagc	agattgtact	gagagtgcac	catatatgcg	gtgtgaaata	3800
3801	ccgcacagat	gcgtaaggag	aaaataccgc	atcaggcgct	cttccgcttc	3850
3851	ctcgctcact	gactcgctgc	gctcggtcgt	tcggctgcgg	cgagcggtat	3900
3901	cagctcactc	aaaggcggta	atacggttat	ccacagaatc	aggggataac	3950
3951	gcaggaaaga	acatgtgagc	aaaaggccag	caaaaggcca	ggaaccgtaa	4000
4001	aaaggccgcg	ttgctggcgt	ttttccatag	gctccgcccc	cctgacgagc	4050
4051	atcacaaaaa	tcgacgctca	agtcagaggt	ggcgaaaccc	gacaggacta	4100
4101	taaagatacc	aggcgtttcc	ccctggaagc	tccctcgtgc	gctctcctgt	4150
4151	tccgaccctg	ccgcttaccg	gatacctgtc	cgcctttctc	ccttcgggaa	4200
4201	gcgtggcgct	ttctcatagc	tcacgctgta	ggtatctcag	ttcggtgtag	4250
4251	gtcgttcgct	ccaagctggg	ctgtgtgcac	gaaccccccg	ttcagcccga	4300
4301	ccgctgcgcc	ttatccggta	actatcgtct	tgagtccaac	ccggtaagac	4350
4351	acgacttatc	gccactggca	gcagccactg	gtaacaggat	tagcagagcg	4400
4401	aggtatgtag	gcggtgctac	agagttcttg	aagtggtggc	ctaactacgg	4450
4451	ctacactaga	aggacagtat	ttggtatctg	cgctctgctg	aagccagtta	4500
4501	ccttcggaaa	aagagttggt	agctcttgat	ccggcaaaca	aaccaccgct	4550
4551	ggtagcggtg	gtttttttgt	ttgcaagcag	cagattacgc	gcagaaaaaa	4600
4601	aggatctcaa	gaagatcctt	tgatcttttc	tacggggtct	gacgctcagt	4650
4651	ggaacgaaaa	ctcacgttaa	gggattttgg	tcatgaacaa	taaaactgtc	4700
4701	tgcttacata	aacagtaata	caaggggtgt	tatgagccat	attcaacggg	4750
4751	aaacgtcttg	ctctaggccg	cgattaaatt	ccaacatgga	tgctgattta	4800
4801	tatgggtata	aatgggctcg	cgataatgtc	gggcaatcag	gtgcgacaat	4850
4851	ctatcgattg	tatgggaagc	ccgatgcgcc	agagttgttt	ctgaaacatg	4900
4901	gcaaaggtag	cgttgccaat	gatgttacag	atgagatggt	cagactaaac	4950
4951	tggctgacgg	aatttatgcc	tcttccgacc	atcaagcatt	ttatccgtac	5000
5001	tcctgatgat	gcatggttac	tcaccactgc	gatccccggg	aaaacagcat	5050
5051	tccaggtatt	agaagaatat	cctgattcag	gtgaaaatat	tgttgatgcg	5100
5101	ctggcagtgt	tcctgcgccg	gttgcattcg	attcctgttt	gtaattgtcc	5150
5151	ttttaacagc	gatcgcgtat	ttcgtctcgc	tcaggcgcaa	tcacgaatga	5200
5201	ataacggttt	ggttgatgcg	agtgattttg	atgacgagcg	taatggctgg	5250
5251	cctgttgaac	aagtctggaa	agaaatgcat	aaacttttgc	cattctcacc	5300
5301	ggattcagtc	gtcactcatg	gtgatttctc	acttgataac	cttatttttg	5350
5351	acgaggggaa	attaataggt	tgtattgatg	ttggacgagt	cggaatcgca	5400
5401	gaccgatacc	aggatcttgc	catcctatgg	aactgcctcg	gtgagttttc	5450
5451	tccttcatta	cagaaacggc	tttttcaaaa	atatggtatt	gataatcctg	5500
5501	atatgaataa	attgcagttt	catttgatgc	tcgatgagtt	tttctaagaa	5550
5551	ttaattcatg	agcggataca	tatttgaatg	tatttagaaa	aataaacaaa	5600
5601	taggggttcc	gcgcacattt	ccccgaaaag	tgccacctga	aattgtaaac	5650
5651	gttaatattt	tgttaaaatt	cgcgttaaat	ttttgttaaa	tcagctcatt	5700
5701	ttttaaccaa	taggccgaaa	tcggcaaaat	cccttataaa	tcaaaagaat	5750
5751	agaccgagat	agggttgagt	gttgttccag	tttggaacaa	gagtccacta	5800
5801	ttaaagaacg	tggactccaa	cgtcaaaggg	cgaaaaaccg	tctatcaggg	5850
5851	cgatggccca	ctacgtgaac	catcacccta	atcaagtttt	ttggggtcga	5900
5901	ggtgccgtaa	agcactaaat	cggaacccta	aagggagccc	ccgatttaga	5950
5951	gcttgacggg	gaaagccggc	gaacgtggcg	agaaaggaag	ggaagaaagc	6000
6001	gaaaggagcg	ggcgctaggg	cgctggcaag	tgtagcggtc	acgctgcgcg	6050
6051	taaccaccac	acccgccgcg	cttaatgcgc	cgctacaggg	cgcgtcccat	6100
6101	tcgcca	6106