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低温运输,-20℃保存
1年
pET-33b(+)
大量
信裕生物
名称 | 编号 | 规格 | 价格 | 手册 |
---|---|---|---|---|
pET-33b(+)(pET33b)(60908-2802)(1000-1) | 60908-2802 |
质粒编号: 60908-2802
质粒名称: pET-33b(+)
其他名称:
质粒大小(bp): 5383
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: His (中间和C端), N-Thrombin
5’测序引物:T7
5′ 测序引物序列:T7: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Production of target proteins suitable for site-specific 32P-labeling; Nterm thrombin cleavage site; Nterm enterokinase cleavage site.
质粒图谱:
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
T7_leader | 238 | 206 | |
Xpress_fwd_primer | 239 | 221 | |
T7_transl_en_RBS | 337 | 321 | |
lacO | 382 | 355 | |
T7_promoter | 400 | 382 | |
tet (300 – 563) | 432 | 695 | |
pBRrevBam_primer | 503 | 484 | |
lacI | 778 | 1869 | |
tet (576 – 851) | 1928 | 2203 | |
ROP | 2678 | 2869 | |
pGEX_3_primer | 2885 | 2863 | |
pBR322_origin | 3903 | 3284 | |
KanR2 | 4009 | 4824 |
ORF | Start | End | |
---|---|---|---|
ORF frame 3 | 537 | 1037 | |
ORF frame 1 | 910 | 1869 | |
ORF frame 1 | 4009 | 4824 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NheI | 230 |
NdeI | 237 |
NcoI | 310 |
XbaI | 349 |
BglII | 415 |
BclI | 1151 |
ApaI | 1348 |
EcoRV | 1587 |
HpaI | 1643 |
FspI | 2219 |
NruI | 4097 |
ClaI | 4131 |
XmaI | 4312 |
SmaI | 4314 |
质粒序列:
1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
201 | tcccgaccca | tttgctgtcc | accagtcatg | ctagccatat | gaacagatgc | 250 |
251 | acgacggctg | ccgcgcggca | ccaggccgct | gctgtgatga | tgatgatgat | 300 |
301 | ggctgctgcc | catggtatat | ctccttctta | aagttaaaca | aaattatttc | 350 |
351 | tagaggggaa | ttgttatccg | ctcacaattc | ccctatagtg | agtcgtatta | 400 |
401 | atttcgcggg | atcgagatct | cgatcctcta | cgccggacgc | atcgtggccg | 450 |
451 | gcatcaccgg | cgccacaggt | gcggttgctg | gcgcctatat | cgccgacatc | 500 |
501 | accgatgggg | aagatcgggc | tcgccacttc | gggctcatga | gcgcttgttt | 550 |
551 | cggcgtgggt | atggtggcag | gccccgtggc | cgggggactg | ttgggcgcca | 600 |
601 | tctccttgca | tgcaccattc | cttgcggcgg | cggtgctcaa | cggcctcaac | 650 |
651 | ctactactgg | gctgcttcct | aatgcaggag | tcgcataagg | gagagcgtcg | 700 |
701 | agatcccgga | caccatcgaa | tggcgcaaaa | cctttcgcgg | tatggcatga | 750 |
751 | tagcgcccgg | aagagagtca | attcagggtg | gtgaatgtga | aaccagtaac | 800 |
801 | gttatacgat | gtcgcagagt | atgccggtgt | ctcttatcag | accgtttccc | 850 |
851 | gcgtggtgaa | ccaggccagc | cacgtttctg | cgaaaacgcg | ggaaaaagtg | 900 |
901 | gaagcggcga | tggcggagct | gaattacatt | cccaaccgcg | tggcacaaca | 950 |
951 | actggcgggc | aaacagtcgt | tgctgattgg | cgttgccacc | tccagtctgg | 1000 |
1001 | ccctgcacgc | gccgtcgcaa | attgtcgcgg | cgattaaatc | tcgcgccgat | 1050 |
1051 | caactgggtg | ccagcgtggt | ggtgtcgatg | gtagaacgaa | gcggcgtcga | 1100 |
1101 | agcctgtaaa | gcggcggtgc | acaatcttct | cgcgcaacgc | gtcagtgggc | 1150 |
1151 | tgatcattaa | ctatccgctg | gatgaccagg | atgccattgc | tgtggaagct | 1200 |
1201 | gcctgcacta | atgttccggc | gttatttctt | gatgtctctg | accagacacc | 1250 |
1251 | catcaacagt | attattttct | cccatgaaga | cggtacgcga | ctgggcgtgg | 1300 |
1301 | agcatctggt | cgcattgggt | caccagcaaa | tcgcgctgtt | agcgggccca | 1350 |
1351 | ttaagttctg | tctcggcgcg | tctgcgtctg | gctggctggc | ataaatatct | 1400 |
1401 | cactcgcaat | caaattcagc | cgatagcgga | acgggaaggc | gactggagtg | 1450 |
1451 | ccatgtccgg | ttttcaacaa | accatgcaaa | tgctgaatga | gggcatcgtt | 1500 |
1501 | cccactgcga | tgctggttgc | caacgatcag | atggcgctgg | gcgcaatgcg | 1550 |
1551 | cgccattacc | gagtccgggc | tgcgcgttgg | tgcggatatc | tcggtagtgg | 1600 |
1601 | gatacgacga | taccgaagac | agctcatgtt | atatcccgcc | gttaaccacc | 1650 |
1651 | atcaaacagg | attttcgcct | gctggggcaa | accagcgtgg | accgcttgct | 1700 |
1701 | gcaactctct | cagggccagg | cggtgaaggg | caatcagctg | ttgcccgtct | 1750 |
1751 | cactggtgaa | aagaaaaacc | accctggcgc | ccaatacgca | aaccgcctct | 1800 |
1801 | ccccgcgcgt | tggccgattc | attaatgcag | ctggcacgac | aggtttcccg | 1850 |
1851 | actggaaagc | gggcagtgag | cgcaacgcaa | ttaatgtaag | ttagctcact | 1900 |
1901 | cattaggcac | cgggatctcg | accgatgccc | ttgagagcct | tcaacccagt | 1950 |
1951 | cagctccttc | cggtgggcgc | ggggcatgac | tatcgtcgcc | gcacttatga | 2000 |
2001 | ctgtcttctt | tatcatgcaa | ctcgtaggac | aggtgccggc | agcgctctgg | 2050 |
2051 | gtcattttcg | gcgaggaccg | ctttcgctgg | agcgcgacga | tgatcggcct | 2100 |
2101 | gtcgcttgcg | gtattcggaa | tcttgcacgc | cctcgctcaa | gccttcgtca | 2150 |
2151 | ctggtcccgc | caccaaacgt | ttcggcgaga | agcaggccat | tatcgccggc | 2200 |
2201 | atggcggccc | cacgggtgcg | catgatcgtg | ctcctgtcgt | tgaggacccg | 2250 |
2251 | gctaggctgg | cggggttgcc | ttactggtta | gcagaatgaa | tcaccgatac | 2300 |
2301 | gcgagcgaac | gtgaagcgac | tgctgctgca | aaacgtctgc | gacctgagca | 2350 |
2351 | acaacatgaa | tggtcttcgg | tttccgtgtt | tcgtaaagtc | tggaaacgcg | 2400 |
2401 | gaagtcagcg | ccctgcacca | ttatgttccg | gatctgcatc | gcaggatgct | 2450 |
2451 | gctggctacc | ctgtggaaca | cctacatctg | tattaacgaa | gcgctggcat | 2500 |
2501 | tgaccctgag | tgatttttct | ctggtcccgc | cgcatccata | ccgccagttg | 2550 |
2551 | tttaccctca | caacgttcca | gtaaccgggc | atgttcatca | tcagtaaccc | 2600 |
2601 | gtatcgtgag | catcctctct | cgtttcatcg | gtatcattac | ccccatgaac | 2650 |
2651 | agaaatcccc | cttacacgga | ggcatcagtg | accaaacagg | aaaaaaccgc | 2700 |
2701 | ccttaacatg | gcccgcttta | tcagaagcca | gacattaacg | cttctggaga | 2750 |
2751 | aactcaacga | gctggacgcg | gatgaacagg | cagacatctg | tgaatcgctt | 2800 |
2801 | cacgaccacg | ctgatgagct | ttaccgcagc | tgcctcgcgc | gtttcggtga | 2850 |
2851 | tgacggtgaa | aacctctgac | acatgcagct | cccggagacg | gtcacagctt | 2900 |
2901 | gtctgtaagc | ggatgccggg | agcagacaag | cccgtcaggg | cgcgtcagcg | 2950 |
2951 | ggtgttggcg | ggtgtcgggg | cgcagccatg | acccagtcac | gtagcgatag | 3000 |
3001 | cggagtgtat | actggcttaa | ctatgcggca | tcagagcaga | ttgtactgag | 3050 |
3051 | agtgcaccat | atatgcggtg | tgaaataccg | cacagatgcg | taaggagaaa | 3100 |
3101 | ataccgcatc | aggcgctctt | ccgcttcctc | gctcactgac | tcgctgcgct | 3150 |
3151 | cggtcgttcg | gctgcggcga | gcggtatcag | ctcactcaaa | ggcggtaata | 3200 |
3201 | cggttatcca | cagaatcagg | ggataacgca | ggaaagaaca | tgtgagcaaa | 3250 |
3251 | aggccagcaa | aaggccagga | accgtaaaaa | ggccgcgttg | ctggcgtttt | 3300 |
3301 | tccataggct | ccgcccccct | gacgagcatc | acaaaaatcg | acgctcaagt | 3350 |
3351 | cagaggtggc | gaaacccgac | aggactataa | agataccagg | cgtttccccc | 3400 |
3401 | tggaagctcc | ctcgtgcgct | ctcctgttcc | gaccctgccg | cttaccggat | 3450 |
3451 | acctgtccgc | ctttctccct | tcgggaagcg | tggcgctttc | tcatagctca | 3500 |
3501 | cgctgtaggt | atctcagttc | ggtgtaggtc | gttcgctcca | agctgggctg | 3550 |
3551 | tgtgcacgaa | ccccccgttc | agcccgaccg | ctgcgcctta | tccggtaact | 3600 |
3601 | atcgtcttga | gtccaacccg | gtaagacacg | acttatcgcc | actggcagca | 3650 |
3651 | gccactggta | acaggattag | cagagcgagg | tatgtaggcg | gtgctacaga | 3700 |
3701 | gttcttgaag | tggtggccta | actacggcta | cactagaagg | acagtatttg | 3750 |
3751 | gtatctgcgc | tctgctgaag | ccagttacct | tcggaaaaag | agttggtagc | 3800 |
3801 | tcttgatccg | gcaaacaaac | caccgctggt | agcggtggtt | tttttgtttg | 3850 |
3851 | caagcagcag | attacgcgca | gaaaaaaagg | atctcaagaa | gatcctttga | 3900 |
3901 | tcttttctac | ggggtctgac | gctcagtgga | acgaaaactc | acgttaaggg | 3950 |
3951 | attttggtca | tgaacaataa | aactgtctgc | ttacataaac | agtaatacaa | 4000 |
4001 | ggggtgttat | gagccatatt | caacgggaaa | cgtcttgctc | taggccgcga | 4050 |
4051 | ttaaattcca | acatggatgc | tgatttatat | gggtataaat | gggctcgcga | 4100 |
4101 | taatgtcggg | caatcaggtg | cgacaatcta | tcgattgtat | gggaagcccg | 4150 |
4151 | atgcgccaga | gttgtttctg | aaacatggca | aaggtagcgt | tgccaatgat | 4200 |
4201 | gttacagatg | agatggtcag | actaaactgg | ctgacggaat | ttatgcctct | 4250 |
4251 | tccgaccatc | aagcatttta | tccgtactcc | tgatgatgca | tggttactca | 4300 |
4301 | ccactgcgat | ccccgggaaa | acagcattcc | aggtattaga | agaatatcct | 4350 |
4351 | gattcaggtg | aaaatattgt | tgatgcgctg | gcagtgttcc | tgcgccggtt | 4400 |
4401 | gcattcgatt | cctgtttgta | attgtccttt | taacagcgat | cgcgtatttc | 4450 |
4451 | gtctcgctca | ggcgcaatca | cgaatgaata | acggtttggt | tgatgcgagt | 4500 |
4501 | gattttgatg | acgagcgtaa | tggctggcct | gttgaacaag | tctggaaaga | 4550 |
4551 | aatgcataaa | cttttgccat | tctcaccgga | ttcagtcgtc | actcatggtg | 4600 |
4601 | atttctcact | tgataacctt | atttttgacg | aggggaaatt | aataggttgt | 4650 |
4651 | attgatgttg | gacgagtcgg | aatcgcagac | cgataccagg | atcttgccat | 4700 |
4701 | cctatggaac | tgcctcggtg | agttttctcc | ttcattacag | aaacggcttt | 4750 |
4751 | ttcaaaaata | tggtattgat | aatcctgata | tgaataaatt | gcagtttcat | 4800 |
4801 | ttgatgctcg | atgagttttt | ctaagaatta | attcatgagc | ggatacatat | 4850 |
4851 | ttgaatgtat | ttagaaaaat | aaacaaatag | gggttccgcg | cacatttccc | 4900 |
4901 | cgaaaagtgc | cacctgaaat | tgtaaacgtt | aatattttgt | taaaattcgc | 4950 |
4951 | gttaaatttt | tgttaaatca | gctcattttt | taaccaatag | gccgaaatcg | 5000 |
5001 | gcaaaatccc | ttataaatca | aaagaataga | ccgagatagg | gttgagtgtt | 5050 |
5051 | gttccagttt | ggaacaagag | tccactatta | aagaacgtgg | actccaacgt | 5100 |
5101 | caaagggcga | aaaaccgtct | atcagggcga | tggcccacta | cgtgaaccat | 5150 |
5151 | caccctaatc | aagttttttg | gggtcgaggt | gccgtaaagc | actaaatcgg | 5200 |
5201 | aaccctaaag | ggagcccccg | atttagagct | tgacggggaa | agccggcgaa | 5250 |
5251 | cgtggcgaga | aaggaaggga | agaaagcgaa | aggagcgggc | gctagggcgc | 5300 |
5301 | tggcaagtgt | agcggtcacg | ctgcgcgtaa | ccaccacacc | cgccgcgctt | 5350 |
5351 | aatgcgccgc | tacagggcgc | gtcccattcg | cca | 5383 |
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