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- TIANDZ
- 中国
- 60908-2797y
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pET-30a(+)
- 库存：
大量
- 供应商：
信裕生物
质粒编号: 60908-2797y
质粒名称: pET-30a(+)
其他名称: pET30a
质粒大小（bp）: 5422
抗性: Kanamycin (卡那霉素)
质粒类型:大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-S, N-Thrombin, N-EK, C-His
5′ 测序引物及序列:T7: 5′-TAATACGACTCACTATAGGG-3′
3′ 测序引物序列: T7t: 5′-GCTAGTTATTGCTCAGCGG-3′
GenBank号:
其他属性:
Notes Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS
质粒图谱:
质粒序列:
| 1 | ATCCGGATAT | AGTTCCTCCT | TTCAGCAAAA | AACCCCTCAA | GACCCGTTTA | 50 |
| 51 | GAGGCCCCAA | GGGGTTATGC | TAGTTATTGC | TCAGCGGTGG | CAGCAGCCAA | 100 |
| 101 | CTCAGCTTCC | TTTCGGGCTT | TGTTAGCAGC | CGGATCTCAG | TGGTGGTGGT | 150 |
| 151 | GGTGGTGCTC | GAGTGCGGCC | GCAAGCTTGT | CGACGGAGCT | CGAATTCGGA | 200 |
| 201 | TCCGATATCA | GCCATGGCCT | TGTCGTCGTC | GTCGGTACCC | AGATCTGGGC | 250 |
| 251 | TGTCCATGTG | CTGGCGTTCG | AATTTAGCAG | CAGCGGTTTC | TTTCATACCA | 300 |
| 301 | GAACCGCGTG | GCACCAGACC | AGAAGAATGA | TGATGATGAT | GGTGCATATG | 350 |
| 351 | TATATCTCCT | TCTTAAAGTT | AAACAAAATT | ATTTCTAGAG | GGGAATTGTT | 400 |
| 401 | ATCCGCTCAC | AATTCCCCTA | TAGTGAGTCG | TATTAATTTC | GCGGGATCGA | 450 |
| 451 | GATCGATCTC | GATCCTCTAC | GCCGGACGCA | TCGTGGCCGG | CATCACCGGC | 500 |
| 501 | GCCACAGGTG | CGGTTGCTGG | CGCCTATATC | GCCGACATCA | CCGATGGGGA | 550 |
| 551 | AGATCGGGCT | CGCCACTTCG | GGCTCATGAG | CGCTTGTTTC | GGCGTGGGTA | 600 |
| 601 | TGGTGGCAGG | CCCCGTGGCC | GGGGGACTGT | TGGGCGCCAT | CTCCTTGCAT | 650 |
| 651 | GCACCATTCC | TTGCGGCGGC | GGTGCTCAAC | GGCCTCAACC | TACTACTGGG | 700 |
| 701 | CTGCTTCCTA | ATGCAGGAGT | CGCATAAGGG | AGAGCGTCGA | GATCCCGGAC | 750 |
| 751 | ACCATCGAAT | GGCGCAAAAC | CTTTCGCGGT | ATGGCATGAT | AGCGCCCGGA | 800 |
| 801 | AGAGAGTCAA | TTCAGGGTGG | TGAATGTGAA | ACCAGTAACG | TTATACGATG | 850 |
| 851 | TCGCAGAGTA | TGCCGGTGTC | TCTTATCAGA | CCGTTTCCCG | CGTGGTGAAC | 900 |
| 901 | CAGGCCAGCC | ACGTTTCTGC | GAAAACGCGG | GAAAAAGTGG | AAGCGGCGAT | 950 |
| 951 | GGCGGAGCTG | AATTACATTC | CCAACCGCGT | GGCACAACAA | CTGGCGGGCA | 1000 |
| 1001 | AACAGTCGTT | GCTGATTGGC | GTTGCCACCT | CCAGTCTGGC | CCTGCACGCG | 1050 |
| 1051 | CCGTCGCAAA | TTGTCGCGGC | GATTAAATCT | CGCGCCGATC | AACTGGGTGC | 1100 |
| 1101 | CAGCGTGGTG | GTGTCGATGG | TAGAACGAAG | CGGCGTCGAA | GCCTGTAAAG | 1150 |
| 1151 | CGGCGGTGCA | CAATCTTCTC | GCGCAACGCG | TCAGTGGGCT | GATCATTAAC | 1200 |
| 1201 | TATCCGCTGG | ATGACCAGGA | TGCCATTGCT | GTGGAAGCTG | CCTGCACTAA | 1250 |
| 1251 | TGTTCCGGCG | TTATTTCTTG | ATGTCTCTGA | CCAGACACCC | ATCAACAGTA | 1300 |
| 1301 | TTATTTTCTC | CCATGAAGAC | GGTACGCGAC | TGGGCGTGGA | GCATCTGGTC | 1350 |
| 1351 | GCATTGGGTC | ACCAGCAAAT | CGCGCTGTTA | GCGGGCCCAT | TAAGTTCTGT | 1400 |
| 1401 | CTCGGCGCGT | CTGCGTCTGG | CTGGCTGGCA | TAAATATCTC | ACTCGCAATC | 1450 |
| 1451 | AAATTCAGCC | GATAGCGGAA | CGGGAAGGCG | ACTGGAGTGC | CATGTCCGGT | 1500 |
| 1501 | TTTCAACAAA | CCATGCAAAT | GCTGAATGAG | GGCATCGTTC | CCACTGCGAT | 1550 |
| 1551 | GCTGGTTGCC | AACGATCAGA | TGGCGCTGGG | CGCAATGCGC | GCCATTACCG | 1600 |
| 1601 | AGTCCGGGCT | GCGCGTTGGT | GCGGACATCT | CGGTAGTGGG | ATACGACGAT | 1650 |
| 1651 | ACCGAAGACA | GCTCATGTTA | TATCCCGCCG | TTAACCACCA | TCAAACAGGA | 1700 |
| 1701 | TTTTCGCCTG | CTGGGGCAAA | CCAGCGTGGA | CCGCTTGCTG | CAACTCTCTC | 1750 |
| 1751 | AGGGCCAGGC | GGTGAAGGGC | AATCAGCTGT | TGCCCGTCTC | ACTGGTGAAA | 1800 |
| 1801 | AGAAAAACCA | CCCTGGCGCC | CAATACGCAA | ACCGCCTCTC | CCCGCGCGTT | 1850 |
| 1851 | GGCCGATTCA | TTAATGCAGC | TGGCACGACA | GGTTTCCCGA | CTGGAAAGCG | 1900 |
| 1901 | GGCAGTGAGC | GCAACGCAAT | TAATGTAAGT | TAGCTCACTC | ATTAGGCACC | 1950 |
| 1951 | GGGATCTCGA | CCGATGCCCT | TGAGAGCCTT | CAACCCAGTC | AGCTCCTTCC | 2000 |
| 2001 | GGTGGGCGCG | GGGCATGACT | ATCGTCGCCG | CACTTATGAC | TGTCTTCTTT | 2050 |
| 2051 | ATCATGCAAC | TCGTAGGACA | GGTGCCGGCA | GCGCTCTGGG | TCATTTTCGG | 2100 |
| 2101 | CGAGGACCGC | TTTCGCTGGA | GCGCGACGAT | GATCGGCCTG | TCGCTTGCGG | 2150 |
| 2151 | TATTCGGAAT | CTTGCACGCC | CTCGCTCAAG | CCTTCGTCAC | TGGTCCCGCC | 2200 |
| 2201 | ACCAAACGTT | TCGGCGAGAA | GCAGGCCATT | ATCGCCGGCA | TGGCGGCCCC | 2250 |
| 2251 | ACGGGTGCGC | ATGATCGTGC | TCCTGTCGTT | GAGGACCCGG | CTAGGCTGGC | 2300 |
| 2301 | GGGGTTGCCT | TACTGGTTAG | CAGAATGAAT | CACCGATACG | CGAGCGAACG | 2350 |
| 2351 | TGAAGCGACT | GCTGCTGCAA | AACGTCTGCG | ACCTGAGCAA | CAACATGAAT | 2400 |
| 2401 | GGTCTTCGGT | TTCCGTGTTT | CGTAAAGTCT | GGAAACGCGG | AAGTCAGCGC | 2450 |
| 2451 | CCTGCACCAT | TATGTTCCGG | ATCTGCATCG | CAGGATGCTG | CTGGCTACCC | 2500 |
| 2501 | TGTGGAACAC | CTACATCTGT | ATTAACGAAG | CGCTGGCATT | GACCCTGAGT | 2550 |
| 2551 | GATTTTTCTC | TGGTCCCGCC | GCATCCATAC | CGCCAGTTGT | TTACCCTCAC | 2600 |
| 2601 | AACGTTCCAG | TAACCGGGCA | TGTTCATCAT | CAGTAACCCG | TATCGTGAGC | 2650 |
| 2651 | ATCCTCTCTC | GTTTCATCGG | TATCATTACC | CCCATGAACA | GAAATCCCCC | 2700 |
| 2701 | TTACACGGAG | GCATCAGTGA | CCAAACAGGA | AAAAACCGCC | CTTAACATGG | 2750 |
| 2751 | CCCGCTTTAT | CAGAAGCCAG | ACATTAACGC | TTCTGGAGAA | ACTCAACGAG | 2800 |
| 2801 | CTGGACGCGG | ATGAACAGGC | AGACATCTGT | GAATCGCTTC | ACGACCACGC | 2850 |
| 2851 | TGATGAGCTT | TACCGCAGCT | GCCTCGCGCG | TTTCGGTGAT | GACGGTGAAA | 2900 |
| 2901 | ACCTCTGACA | CATGCAGCTC | CCGGAGACGG | TCACAGCTTG | TCTGTAAGCG | 2950 |
| 2951 | GATGCCGGGA | GCAGACAAGC | CCGTCAGGGC | GCGTCAGCGG | GTGTTGGCGG | 3000 |
| 3001 | GTGTCGGGGC | GCAGCCATGA | CCCAGTCACG | TAGCGATAGC | GGAGTGTATA | 3050 |
| 3051 | CTGGCTTAAC | TATGCGGCAT | CAGAGCAGAT | TGTACTGAGA | GTGCACCATA | 3100 |
| 3101 | TATGCGGTGT | GAAATACCGC | ACAGATGCGT | AAGGAGAAAA | TACCGCATCA | 3150 |
| 3151 | GGCGCTCTTC | CGCTTCCTCG | CTCACTGACT | CGCTGCGCTC | GGTCGTTCGG | 3200 |
| 3201 | CTGCGGCGAG | CGGTATCAGC | TCACTCAAAG | GCGGTAATAC | GGTTATCCAC | 3250 |
| 3251 | AGAATCAGGG | GATAACGCAG | GAAAGAACAT | GTGAGCAAAA | GGCCAGCAAA | 3300 |
| 3301 | AGGCCAGGAA | CCGTAAAAAG | GCCGCGTTGC | TGGCGTTTTT | CCATAGGCTC | 3350 |
| 3351 | CGCCCCCCTG | ACGAGCATCA | CAAAAATCGA | CGCTCAAGTC | AGAGGTGGCG | 3400 |
| 3401 | AAACCCGACA | GGACTATAAA | GATACCAGGC | GTTTCCCCCT | GGAAGCTCCC | 3450 |
| 3451 | TCGTGCGCTC | TCCTGTTCCG | ACCCTGCCGC | TTACCGGATA | CCTGTCCGCC | 3500 |
| 3501 | TTTCTCCCTT | CGGGAAGCGT | GGCGCTTTCT | CATAGCTCAC | GCTGTAGGTA | 3550 |
| 3551 | TCTCAGTTCG | GTGTAGGTCG | TTCGCTCCAA | GCTGGGCTGT | GTGCACGAAC | 3600 |
| 3601 | CCCCCGTTCA | GCCCGACCGC | TGCGCCTTAT | CCGGTAACTA | TCGTCTTGAG | 3650 |
| 3651 | TCCAACCCGG | TAAGACACGA | CTTATCGCCA | CTGGCAGCAG | CCACTGGTAA | 3700 |
| 3701 | CAGGATTAGC | AGAGCGAGGT | ATGTAGGCGG | TGCTACAGAG | TTCTTGAAGT | 3750 |
| 3751 | GGTGGCCTAA | CTACGGCTAC | ACTAGAAGGA | CAGTATTTGG | TATCTGCGCT | 3800 |
| 3801 | CTGCTGAAGC | CAGTTACCTT | CGGAAAAAGA | GTTGGTAGCT | CTTGATCCGG | 3850 |
| 3851 | CAAACAAACC | ACCGCTGGTA | GCGGTGGTTT | TTTTGTTTGC | AAGCAGCAGA | 3900 |
| 3901 | TTACGCGCAG | AAAAAAAGGA | TCTCAAGAAG | ATCCTTTGAT | CTTTTCTACG | 3950 |
| 3951 | GGGTCTGACG | CTCAGTGGAA | CGAAAACTCA | CGTTAAGGGA | TTTTGGTCAT | 4000 |
| 4001 | GAACAATAAA | ACTGTCTGCT | TACATAAACA | GTAATACAAG | GGGTGTTATG | 4050 |
| 4051 | AGCCATATTC | AACGGGAAAC | GTCTTGCTCT | AGGCCGCGAT | TAAATTCCAA | 4100 |
| 4101 | CATGGATGCT | GATTTATATG | GGTATAAATG | GGCTCGCGAT | AATGTCGGGC | 4150 |
| 4151 | AATCAGGTGC | GACAATCTAT | CGATTGTATG | GGAAGCCCGA | TGCGCCAGAG | 4200 |
| 4201 | TTGTTTCTGA | AACATGGCAA | AGGTAGCGTT | GCCAATGATG | TTACAGATGA | 4250 |
| 4251 | GATGGTCAGA | CTAAACTGGC | TGACGGAATT | TATGCCTCTT | CCGACCATCA | 4300 |
| 4301 | AGCATTTTAT | CCGTACTCCT | GATGATGCAT | GGTTACTCAC | CACTGCGATC | 4350 |
| 4351 | CCCGGGAAAA | CAGCATTCCA | GGTATTAGAA | GAATATCCTG | ATTCAGGTGA | 4400 |
| 4401 | AAATATTGTT | GATGCGCTGG | CAGTGTTCCT | GCGCCGGTTG | CATTCGATTC | 4450 |
| 4451 | CTGTTTGTAA | TTGTCCTTTT | AACAGCGATC | GCGTATTTCG | TCTCGCTCAG | 4500 |
| 4501 | GCGCAATCAC | GAATGAATAA | CGGTTTGGTT | GATGCGAGTG | ATTTTGATGA | 4550 |
| 4551 | CGAGCGTAAT | GGCTGGCCTG | TTGAACAAGT | CTGGAAAGAA | ATGCATAAAC | 4600 |
| 4601 | TTTTGCCATT | CTCACCGGAT | TCAGTCGTCA | CTCATGGTGA | TTTCTCACTT | 4650 |
| 4651 | GATAACCTTA | TTTTTGACGA | GGGGAAATTA | ATAGGTTGTA | TTGATGTTGG | 4700 |
| 4701 | ACGAGTCGGA | ATCGCAGACC | GATACCAGGA | TCTTGCCATC | CTATGGAACT | 4750 |
| 4751 | GCCTCGGTGA | GTTTTCTCCT | TCATTACAGA | AACGGCTTTT | TCAAAAATAT | 4800 |
| 4801 | GGTATTGATA | ATCCTGATAT | GAATAAATTG | CAGTTTCATT | TGATGCTCGA | 4850 |
| 4851 | TGAGTTTTTC | TAAGAATTAA | TTCATGAGCG | GATACATATT | TGAATGTATT | 4900 |
| 4901 | TAGAAAAATA | AACAAATAGG | GGTTCCGCGC | ACATTTCCCC | GAAAAGTGCC | 4950 |
| 4951 | ACCTGAAATT | GTAAACGTTA | ATATTTTGTT | AAAATTCGCG | TTAAATTTTT | 5000 |
| 5001 | GTTAAATCAG | CTCATTTTTT | AACCAATAGG | CCGAAATCGG | CAAAATCCCT | 5050 |
| 5051 | TATAAATCAA | AAGAATAGAC | CGAGATAGGG | TTGAGTGTTG | TTCCAGTTTG | 5100 |
| 5101 | GAACAAGAGT | CCACTATTAA | AGAACGTGGA | CTCCAACGTC | AAAGGGCGAA | 5150 |
| 5151 | AAACCGTCTA | TCAGGGCGAT | GGCCCACTAC | GTGAACCATC | ACCCTAATCA | 5200 |
| 5201 | AGTTTTTTGG | GGTCGAGGTG | CCGTAAAGCA | CTAAATCGGA | ACCCTAAAGG | 5250 |
| 5251 | GAGCCCCCGA | TTTAGAGCTT | GACGGGGAAA | GCCGGCGAAC | GTGGCGAGAA | 5300 |
| 5301 | AGGAAGGGAA | GAAAGCGAAA | GGAGCGGGCG | CTAGGGCGCT | GGCAAGTGTA | 5350 |
| 5351 | GCGGTCACGC | TGCGCGTAAC | CACCACACCC | GCCGCGCTTA | ATGCGCCGCT | 5400 |
| 5401 | ACAGGGCGCG | TCCCATTCGC | CA | 5422 |
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pET-30a(+)
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