pET-28a(+)

名称	编号	规格	价格	手册
pET-28a(+)(pET28a)	60908-2793y

质粒编号: 60908-2793y
质粒名称: pET-28a(+)
其他名称:
质粒大小（bp）: 5369
抗性: Kanamycin
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-T7, C-His
5’测序引物: T7: TAATACGACTCACTATAGGG

3′ 测序引物:T7ter: GCTAGTTATTGCTCAGCGG
GenBank号:
其他属性: Nterm thrombin cleavage site; a,b,c vary by MCS;
质粒图谱:

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
T7_leader	239	207
Xpress_fwd_primer	240	222
T7_transl_en_RBS	323	307
lacO	368	341
T7_promoter	386	368
tet (300 – 563)	418	681
pBRrevBam_primer	489	470
lacI	764	1855
tet (576 – 851)	1914	2189
ROP	2664	2855
pGEX_3_primer	2871	2849
pBR322_origin	3889	3270
KanR2	3995	4810

ORF	Start	End
ORF frame 1	523	1023
ORF frame 2	896	1855
ORF frame 2	3995	4810

Enzyme Name	Cut
XhoI	158
NotI	166
EagI	166
HindIII	173
SalI	179
SacI	190
EcoRI	192
BamHI	198
NheI	231
NdeI	238
NcoI	296
XbaI	335
BglII	401
BclI	1137
ApaI	1334
EcoRV	1573
HpaI	1629
FspI	2205
NruI	4083
ClaI	4117
XmaI	4298
SmaI	4300

质粒序列:

1	ATCCGGATAT	AGTTCCTCCT	TTCAGCAAAA	AACCCCTCAA	GACCCGTTTA	50
51	GAGGCCCCAA	GGGGTTATGC	TAGTTATTGC	TCAGCGGTGG	CAGCAGCCAA	100
101	CTCAGCTTCC	TTTCGGGCTT	TGTTAGCAGC	CGGATCTCAG	TGGTGGTGGT	150
151	GGTGGTGCTC	GAGTGCGGCC	GCAAGCTTGT	CGACGGAGCT	CGAATTCGGA	200
201	TCCGCGACCC	ATTTGCTGTC	CACCAGTCAT	GCTAGCCATA	TGGCTGCCGC	250
251	GCGGCACCAG	GCCGCTGCTG	TGATGATGAT	GATGATGGCT	GCTGCCCATG	300
301	GTATATCTCC	TTCTTAAAGT	TAAACAAAAT	TATTTCTAGA	GGGGAATTGT	350
351	TATCCGCTCA	CAATTCCCCT	ATAGTGAGTC	GTATTAATTT	CGCGGGATCG	400
401	AGATCTCGAT	CCTCTACGCC	GGACGCATCG	TGGCCGGCAT	CACCGGCGCC	450
451	ACAGGTGCGG	TTGCTGGCGC	CTATATCGCC	GACATCACCG	ATGGGGAAGA	500
501	TCGGGCTCGC	CACTTCGGGC	TCATGAGCGC	TTGTTTCGGC	GTGGGTATGG	550
551	TGGCAGGCCC	CGTGGCCGGG	GGACTGTTGG	GCGCCATCTC	CTTGCATGCA	600
601	CCATTCCTTG	CGGCGGCGGT	GCTCAACGGC	CTCAACCTAC	TACTGGGCTG	650
651	CTTCCTAATG	CAGGAGTCGC	ATAAGGGAGA	GCGTCGAGAT	CCCGGACACC	700
701	ATCGAATGGC	GCAAAACCTT	TCGCGGTATG	GCATGATAGC	GCCCGGAAGA	750
751	GAGTCAATTC	AGGGTGGTGA	ATGTGAAACC	AGTAACGTTA	TACGATGTCG	800
801	CAGAGTATGC	CGGTGTCTCT	TATCAGACCG	TTTCCCGCGT	GGTGAACCAG	850
851	GCCAGCCACG	TTTCTGCGAA	AACGCGGGAA	AAAGTGGAAG	CGGCGATGGC	900
901	GGAGCTGAAT	TACATTCCCA	ACCGCGTGGC	ACAACAACTG	GCGGGCAAAC	950
951	AGTCGTTGCT	GATTGGCGTT	GCCACCTCCA	GTCTGGCCCT	GCACGCGCCG	1000
1001	TCGCAAATTG	TCGCGGCGAT	TAAATCTCGC	GCCGATCAAC	TGGGTGCCAG	1050
1051	CGTGGTGGTG	TCGATGGTAG	AACGAAGCGG	CGTCGAAGCC	TGTAAAGCGG	1100
1101	CGGTGCACAA	TCTTCTCGCG	CAACGCGTCA	GTGGGCTGAT	CATTAACTAT	1150
1151	CCGCTGGATG	ACCAGGATGC	CATTGCTGTG	GAAGCTGCCT	GCACTAATGT	1200
1201	TCCGGCGTTA	TTTCTTGATG	TCTCTGACCA	GACACCCATC	AACAGTATTA	1250
1251	TTTTCTCCCA	TGAAGACGGT	ACGCGACTGG	GCGTGGAGCA	TCTGGTCGCA	1300
1301	TTGGGTCACC	AGCAAATCGC	GCTGTTAGCG	GGCCCATTAA	GTTCTGTCTC	1350
1351	GGCGCGTCTG	CGTCTGGCTG	GCTGGCATAA	ATATCTCACT	CGCAATCAAA	1400
1401	TTCAGCCGAT	AGCGGAACGG	GAAGGCGACT	GGAGTGCCAT	GTCCGGTTTT	1450
1451	CAACAAACCA	TGCAAATGCT	GAATGAGGGC	ATCGTTCCCA	CTGCGATGCT	1500
1501	GGTTGCCAAC	GATCAGATGG	CGCTGGGCGC	AATGCGCGCC	ATTACCGAGT	1550
1551	CCGGGCTGCG	CGTTGGTGCG	GATATCTCGG	TAGTGGGATA	CGACGATACC	1600
1601	GAAGACAGCT	CATGTTATAT	CCCGCCGTTA	ACCACCATCA	AACAGGATTT	1650
1651	TCGCCTGCTG	GGGCAAACCA	GCGTGGACCG	CTTGCTGCAA	CTCTCTCAGG	1700
1701	GCCAGGCGGT	GAAGGGCAAT	CAGCTGTTGC	CCGTCTCACT	GGTGAAAAGA	1750
1751	AAAACCACCC	TGGCGCCCAA	TACGCAAACC	GCCTCTCCCC	GCGCGTTGGC	1800
1801	CGATTCATTA	ATGCAGCTGG	CACGACAGGT	TTCCCGACTG	GAAAGCGGGC	1850
1851	AGTGAGCGCA	ACGCAATTAA	TGTAAGTTAG	CTCACTCATT	AGGCACCGGG	1900
1901	ATCTCGACCG	ATGCCCTTGA	GAGCCTTCAA	CCCAGTCAGC	TCCTTCCGGT	1950
1951	GGGCGCGGGG	CATGACTATC	GTCGCCGCAC	TTATGACTGT	CTTCTTTATC	2000
2001	ATGCAACTCG	TAGGACAGGT	GCCGGCAGCG	CTCTGGGTCA	TTTTCGGCGA	2050
2051	GGACCGCTTT	CGCTGGAGCG	CGACGATGAT	CGGCCTGTCG	CTTGCGGTAT	2100
2101	TCGGAATCTT	GCACGCCCTC	GCTCAAGCCT	TCGTCACTGG	TCCCGCCACC	2150
2151	AAACGTTTCG	GCGAGAAGCA	GGCCATTATC	GCCGGCATGG	CGGCCCCACG	2200
2201	GGTGCGCATG	ATCGTGCTCC	TGTCGTTGAG	GACCCGGCTA	GGCTGGCGGG	2250
2251	GTTGCCTTAC	TGGTTAGCAG	AATGAATCAC	CGATACGCGA	GCGAACGTGA	2300
2301	AGCGACTGCT	GCTGCAAAAC	GTCTGCGACC	TGAGCAACAA	CATGAATGGT	2350
2351	CTTCGGTTTC	CGTGTTTCGT	AAAGTCTGGA	AACGCGGAAG	TCAGCGCCCT	2400
2401	GCACCATTAT	GTTCCGGATC	TGCATCGCAG	GATGCTGCTG	GCTACCCTGT	2450
2451	GGAACACCTA	CATCTGTATT	AACGAAGCGC	TGGCATTGAC	CCTGAGTGAT	2500
2501	TTTTCTCTGG	TCCCGCCGCA	TCCATACCGC	CAGTTGTTTA	CCCTCACAAC	2550
2551	GTTCCAGTAA	CCGGGCATGT	TCATCATCAG	TAACCCGTAT	CGTGAGCATC	2600
2601	CTCTCTCGTT	TCATCGGTAT	CATTACCCCC	ATGAACAGAA	ATCCCCCTTA	2650
2651	CACGGAGGCA	TCAGTGACCA	AACAGGAAAA	AACCGCCCTT	AACATGGCCC	2700
2701	GCTTTATCAG	AAGCCAGACA	TTAACGCTTC	TGGAGAAACT	CAACGAGCTG	2750
2751	GACGCGGATG	AACAGGCAGA	CATCTGTGAA	TCGCTTCACG	ACCACGCTGA	2800
2801	TGAGCTTTAC	CGCAGCTGCC	TCGCGCGTTT	CGGTGATGAC	GGTGAAAACC	2850
2851	TCTGACACAT	GCAGCTCCCG	GAGACGGTCA	CAGCTTGTCT	GTAAGCGGAT	2900
2901	GCCGGGAGCA	GACAAGCCCG	TCAGGGCGCG	TCAGCGGGTG	TTGGCGGGTG	2950
2951	TCGGGGCGCA	GCCATGACCC	AGTCACGTAG	CGATAGCGGA	GTGTATACTG	3000
3001	GCTTAACTAT	GCGGCATCAG	AGCAGATTGT	ACTGAGAGTG	CACCATATAT	3050
3051	GCGGTGTGAA	ATACCGCACA	GATGCGTAAG	GAGAAAATAC	CGCATCAGGC	3100
3101	GCTCTTCCGC	TTCCTCGCTC	ACTGACTCGC	TGCGCTCGGT	CGTTCGGCTG	3150
3151	CGGCGAGCGG	TATCAGCTCA	CTCAAAGGCG	GTAATACGGT	TATCCACAGA	3200
3201	ATCAGGGGAT	AACGCAGGAA	AGAACATGTG	AGCAAAAGGC	CAGCAAAAGG	3250
3251	CCAGGAACCG	TAAAAAGGCC	GCGTTGCTGG	CGTTTTTCCA	TAGGCTCCGC	3300
3301	CCCCCTGACG	AGCATCACAA	AAATCGACGC	TCAAGTCAGA	GGTGGCGAAA	3350
3351	CCCGACAGGA	CTATAAAGAT	ACCAGGCGTT	TCCCCCTGGA	AGCTCCCTCG	3400
3401	TGCGCTCTCC	TGTTCCGACC	CTGCCGCTTA	CCGGATACCT	GTCCGCCTTT	3450
3451	CTCCCTTCGG	GAAGCGTGGC	GCTTTCTCAT	AGCTCACGCT	GTAGGTATCT	3500
3501	CAGTTCGGTG	TAGGTCGTTC	GCTCCAAGCT	GGGCTGTGTG	CACGAACCCC	3550
3551	CCGTTCAGCC	CGACCGCTGC	GCCTTATCCG	GTAACTATCG	TCTTGAGTCC	3600
3601	AACCCGGTAA	GACACGACTT	ATCGCCACTG	GCAGCAGCCA	CTGGTAACAG	3650
3651	GATTAGCAGA	GCGAGGTATG	TAGGCGGTGC	TACAGAGTTC	TTGAAGTGGT	3700
3701	GGCCTAACTA	CGGCTACACT	AGAAGGACAG	TATTTGGTAT	CTGCGCTCTG	3750
3751	CTGAAGCCAG	TTACCTTCGG	AAAAAGAGTT	GGTAGCTCTT	GATCCGGCAA	3800
3801	ACAAACCACC	GCTGGTAGCG	GTGGTTTTTT	TGTTTGCAAG	CAGCAGATTA	3850
3851	CGCGCAGAAA	AAAAGGATCT	CAAGAAGATC	CTTTGATCTT	TTCTACGGGG	3900
3901	TCTGACGCTC	AGTGGAACGA	AAACTCACGT	TAAGGGATTT	TGGTCATGAA	3950
3951	CAATAAAACT	GTCTGCTTAC	ATAAACAGTA	ATACAAGGGG	TGTTATGAGC	4000
4001	CATATTCAAC	GGGAAACGTC	TTGCTCTAGG	CCGCGATTAA	ATTCCAACAT	4050
4051	GGATGCTGAT	TTATATGGGT	ATAAATGGGC	TCGCGATAAT	GTCGGGCAAT	4100
4101	CAGGTGCGAC	AATCTATCGA	TTGTATGGGA	AGCCCGATGC	GCCAGAGTTG	4150
4151	TTTCTGAAAC	ATGGCAAAGG	TAGCGTTGCC	AATGATGTTA	CAGATGAGAT	4200
4201	GGTCAGACTA	AACTGGCTGA	CGGAATTTAT	GCCTCTTCCG	ACCATCAAGC	4250
4251	ATTTTATCCG	TACTCCTGAT	GATGCATGGT	TACTCACCAC	TGCGATCCCC	4300
4301	GGGAAAACAG	CATTCCAGGT	ATTAGAAGAA	TATCCTGATT	CAGGTGAAAA	4350
4351	TATTGTTGAT	GCGCTGGCAG	TGTTCCTGCG	CCGGTTGCAT	TCGATTCCTG	4400
4401	TTTGTAATTG	TCCTTTTAAC	AGCGATCGCG	TATTTCGTCT	CGCTCAGGCG	4450
4451	CAATCACGAA	TGAATAACGG	TTTGGTTGAT	GCGAGTGATT	TTGATGACGA	4500
4501	GCGTAATGGC	TGGCCTGTTG	AACAAGTCTG	GAAAGAAATG	CATAAACTTT	4550
4551	TGCCATTCTC	ACCGGATTCA	GTCGTCACTC	ATGGTGATTT	CTCACTTGAT	4600
4601	AACCTTATTT	TTGACGAGGG	GAAATTAATA	GGTTGTATTG	ATGTTGGACG	4650
4651	AGTCGGAATC	GCAGACCGAT	ACCAGGATCT	TGCCATCCTA	TGGAACTGCC	4700
4701	TCGGTGAGTT	TTCTCCTTCA	TTACAGAAAC	GGCTTTTTCA	AAAATATGGT	4750
4751	ATTGATAATC	CTGATATGAA	TAAATTGCAG	TTTCATTTGA	TGCTCGATGA	4800
4801	GTTTTTCTAA	GAATTAATTC	ATGAGCGGAT	ACATATTTGA	ATGTATTTAG	4850
4851	AAAAATAAAC	AAATAGGGGT	TCCGCGCACA	TTTCCCCGAA	AAGTGCCACC	4900
4901	TGAAATTGTA	AACGTTAATA	TTTTGTTAAA	ATTCGCGTTA	AATTTTTGTT	4950
4951	AAATCAGCTC	ATTTTTTAAC	CAATAGGCCG	AAATCGGCAA	AATCCCTTAT	5000
5001	AAATCAAAAG	AATAGACCGA	GATAGGGTTG	AGTGTTGTTC	CAGTTTGGAA	5050
5051	CAAGAGTCCA	CTATTAAAGA	ACGTGGACTC	CAACGTCAAA	GGGCGAAAAA	5100
5101	CCGTCTATCA	GGGCGATGGC	CCACTACGTG	AACCATCACC	CTAATCAAGT	5150
5151	TTTTTGGGGT	CGAGGTGCCG	TAAAGCACTA	AATCGGAACC	CTAAAGGGAG	5200
5201	CCCCCGATTT	AGAGCTTGAC	GGGGAAAGCC	GGCGAACGTG	GCGAGAAAGG	5250
5251	AAGGGAAGAA	AGCGAAAGGA	GCGGGCGCTA	GGGCGCTGGC	AAGTGTAGCG	5300
5301	GTCACGCTGC	GCGTAACCAC	CACACCCGCC	GCGCTTAATG	CGCCGCTACA	5350
5351	GGGCGCGTCC	CATTCGCCA	5369