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- 60908-2774d
- 2025年07月12日
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- 询价记录
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- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pET-11d
- 库存：
大量
- 供应商：
信裕生物
质粒编号: 60908-2774d
质粒名称: pET-11d
其他名称: pET11d
质粒大小（bp）: 5674
抗性: Amp
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7/lacO
稳转或瞬转: 瞬时表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签: N-T7
5’测序引物: T7:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
质粒图谱:
质粒序列:
| 1 | ttctcatgtt | tgacagctta | tcatcgataa | gctttaatgc | ggtagtttat | 50 |
| 51 | cacagttaaa | ttgctaacgc | agtcaggcac | cgtgtatgaa | atctaacaat | 100 |
| 101 | gcgctcatcg | tcatcctcgg | caccgtcacc | ctggatgctg | taggcatagg | 150 |
| 151 | cttggttatg | ccggtactgc | cgggcctctt | gcgggatatc | cggatatagt | 200 |
| 201 | tcctcctttc | agcaaaaaac | ccctcaagac | ccgtttagag | gccccaaggg | 250 |
| 251 | gttatgctag | ttattgctca | gcggtggcag | cagccaactc | agcttccttt | 300 |
| 301 | cgggctttgt | tagcagccgg | atccgaccca | tttgctgtcc | accagtcatg | 350 |
| 351 | ctagccatgg | tatatctcct | tcttaaagtt | aaacaaaatt | atttctagag | 400 |
| 401 | gggaattgtt | atccgctcac | aattccccta | tagtgagtcg | tattaatttc | 450 |
| 451 | gcgggatcga | gatctcgatc | ctctacgccg | gacgcatcgt | ggccggcatc | 500 |
| 501 | accggcgcca | caggtgcggt | tgctggcgcc | tatatcgccg | acatcaccga | 550 |
| 551 | tggggaagat | cgggctcgcc | acttcgggct | catgagcgct | tgtttcggcg | 600 |
| 601 | tgggtatggt | ggcaggcccc | gtggccgggg | gactgttggg | cgccatctcc | 650 |
| 651 | ttgcatgcac | cattccttgc | ggcggcggtg | ctcaacggcc | tcaacctact | 700 |
| 701 | actgggctgc | ttcctaatgc | aggagtcgca | taagggagag | cgtcgagatc | 750 |
| 751 | ccggacacca | tcgaatggcg | caaaaccttt | cgcggtatgg | catgatagcg | 800 |
| 801 | cccggaagag | agtcaattca | gggtggtgaa | tgtgaaacca | gtaacgttat | 850 |
| 851 | acgatgtcgc | agagtatgcc | ggtgtctctt | atcagaccgt | ttcccgcgtg | 900 |
| 901 | gtgaaccagg | ccagccacgt | ttctgcgaaa | acgcgggaaa | aagtggaagc | 950 |
| 951 | ggcgatggcg | gagctgaatt | acattcccaa | ccgcgtggca | caacaactgg | 1000 |
| 1001 | cgggcaaaca | gtcgttgctg | attggcgttg | ccacctccag | tctggccctg | 1050 |
| 1051 | cacgcgccgt | cgcaaattgt | cgcggcgatt | aaatctcgcg | ccgatcaact | 1100 |
| 1101 | gggtgccagc | gtggtggtgt | cgatggtaga | acgaagcggc | gtcgaagcct | 1150 |
| 1151 | gtaaagcggc | ggtgcacaat | cttctcgcgc | aacgcgtcag | tgggctgatc | 1200 |
| 1201 | attaactatc | cgctggatga | ccaggatgcc | attgctgtgg | aagctgcctg | 1250 |
| 1251 | cactaatgtt | ccggcgttat | ttcttgatgt | ctctgaccag | acacccatca | 1300 |
| 1301 | acagtattat | tttctcccat | gaagacggta | cgcgactggg | cgtggagcat | 1350 |
| 1351 | ctggtcgcat | tgggtcacca | gcaaatcgcg | ctgttagcgg | gcccattaag | 1400 |
| 1401 | ttctgtctcg | gcgcgtctgc | gtctggctgg | ctggcataaa | tatctcactc | 1450 |
| 1451 | gcaatcaaat | tcagccgata | gcggaacggg | aaggcgactg | gagtgccatg | 1500 |
| 1501 | tccggttttc | aacaaaccat | gcaaatgctg | aatgagggca | tcgttcccac | 1550 |
| 1551 | tgcgatgctg | gttgccaacg | atcagatggc | gctgggcgca | atgcgcgcca | 1600 |
| 1601 | ttaccgagtc | cgggctgcgc | gttggtgcgg | atatctcggt | agtgggatac | 1650 |
| 1651 | gacgataccg | aagacagctc | atgttatatc | ccgccgttaa | ccaccatcaa | 1700 |
| 1701 | acaggatttt | cgcctgctgg | ggcaaaccag | cgtggaccgc | ttgctgcaac | 1750 |
| 1751 | tctctcaggg | ccaggcggtg | aagggcaatc | agctgttgcc | cgtctcactg | 1800 |
| 1801 | gtgaaaagaa | aaaccaccct | ggcgcccaat | acgcaaaccg | cctctccccg | 1850 |
| 1851 | cgcgttggcc | gattcattaa | tgcagctggc | acgacaggtt | tcccgactgg | 1900 |
| 1901 | aaagcgggca | gtgagcgcaa | cgcaattaat | gtaagttagc | tcactcatta | 1950 |
| 1951 | ggcaccggga | tctcgaccga | tgcccttgag | agccttcaac | ccagtcagct | 2000 |
| 2001 | ccttccggtg | ggcgcggggc | atgactatcg | tcgccgcact | tatgactgtc | 2050 |
| 2051 | ttctttatca | tgcaactcgt | aggacaggtg | ccggcagcgc | tctgggtcat | 2100 |
| 2101 | tttcggcgag | gaccgctttc | gctggagcgc | gacgatgatc | ggcctgtcgc | 2150 |
| 2151 | ttgcggtatt | cggaatcttg | cacgccctcg | ctcaagcctt | cgtcactggt | 2200 |
| 2201 | cccgccacca | aacgtttcgg | cgagaagcag | gccattatcg | ccggcatggc | 2250 |
| 2251 | ggccgacgcg | ctgggctacg | tcttgctggc | gttcgcgacg | cgaggctgga | 2300 |
| 2301 | tggccttccc | cattatgatt | cttctcgctt | ccggcggcat | cgggatgccc | 2350 |
| 2351 | gcgttgcagg | ccatgctgtc | caggcaggta | gatgacgacc | atcagggaca | 2400 |
| 2401 | gcttcaagga | tcgctcgcgg | ctcttaccag | cctaacttcg | atcactggac | 2450 |
| 2451 | cgctgatcgt | cacggcgatt | tatgccgcct | cggcgagcac | atggaacggg | 2500 |
| 2501 | ttggcatgga | ttgtaggcgc | cgccctatac | cttgtctgcc | tccccgcgtt | 2550 |
| 2551 | gcgtcgcggt | gcatggagcc | gggccacctc | gacctgaatg | gaagccggcg | 2600 |
| 2601 | gcacctcgct | aacggattca | ccactccaag | aattggagcc | aatcaattct | 2650 |
| 2651 | tgcggagaac | tgtgaatgcg | caaaccaacc | cttggcagaa | catatccatc | 2700 |
| 2701 | gcgtccgcca | tctccagcag | ccgcacgcgg | cgcatctcgg | gcagcgttgg | 2750 |
| 2751 | gtcctggcca | cgggtgcgca | tgatcgtgct | cctgtcgttg | aggacccggc | 2800 |
| 2801 | taggctggcg | gggttgcctt | actggttagc | agaatgaatc | accgatacgc | 2850 |
| 2851 | gagcgaacgt | gaagcgactg | ctgctgcaaa | acgtctgcga | cctgagcaac | 2900 |
| 2901 | aacatgaatg | gtcttcggtt | tccgtgtttc | gtaaagtctg | gaaacgcgga | 2950 |
| 2951 | agtcagcgcc | ctgcaccatt | atgttccgga | tctgcatcgc | aggatgctgc | 3000 |
| 3001 | tggctaccct | gtggaacacc | tacatctgta | ttaacgaagc | gctggcattg | 3050 |
| 3051 | accctgagtg | atttttctct | ggtcccgccg | catccatacc | gccagttgtt | 3100 |
| 3101 | taccctcaca | acgttccagt | aaccgggcat | gttcatcatc | agtaacccgt | 3150 |
| 3151 | atcgtgagca | tcctctctcg | tttcatcggt | atcattaccc | ccatgaacag | 3200 |
| 3201 | aaatccccct | tacacggagg | catcagtgac | caaacaggaa | aaaaccgccc | 3250 |
| 3251 | ttaacatggc | ccgctttatc | agaagccaga | cattaacgct | tctggagaaa | 3300 |
| 3301 | ctcaacgagc | tggacgcgga | tgaacaggca | gacatctgtg | aatcgcttca | 3350 |
| 3351 | cgaccacgct | gatgagcttt | accgcagctg | cctcgcgcgt | ttcggtgatg | 3400 |
| 3401 | acggtgaaaa | cctctgacac | atgcagctcc | cggagacggt | cacagcttgt | 3450 |
| 3451 | ctgtaagcgg | atgccgggag | cagacaagcc | cgtcagggcg | cgtcagcggg | 3500 |
| 3501 | tgttggcggg | tgtcggggcg | cagccatgac | ccagtcacgt | agcgatagcg | 3550 |
| 3551 | gagtgtatac | tggcttaact | atgcggcatc | agagcagatt | gtactgagag | 3600 |
| 3601 | tgcaccatat | atgcggtgtg | aaataccgca | cagatgcgta | aggagaaaat | 3650 |
| 3651 | accgcatcag | gcgctcttcc | gcttcctcgc | tcactgactc | gctgcgctcg | 3700 |
| 3701 | gtcgttcggc | tgcggcgagc | ggtatcagct | cactcaaagg | cggtaatacg | 3750 |
| 3751 | gttatccaca | gaatcagggg | ataacgcagg | aaagaacatg | tgagcaaaag | 3800 |
| 3801 | gccagcaaaa | ggccaggaac | cgtaaaaagg | ccgcgttgct | ggcgtttttc | 3850 |
| 3851 | cataggctcc | gcccccctga | cgagcatcac | aaaaatcgac | gctcaagtca | 3900 |
| 3901 | gaggtggcga | aacccgacag | gactataaag | ataccaggcg | tttccccctg | 3950 |
| 3951 | gaagctccct | cgtgcgctct | cctgttccga | ccctgccgct | taccggatac | 4000 |
| 4001 | ctgtccgcct | ttctcccttc | gggaagcgtg | gcgctttctc | atagctcacg | 4050 |
| 4051 | ctgtaggtat | ctcagttcgg | tgtaggtcgt | tcgctccaag | ctgggctgtg | 4100 |
| 4101 | tgcacgaacc | ccccgttcag | cccgaccgct | gcgccttatc | cggtaactat | 4150 |
| 4151 | cgtcttgagt | ccaacccggt | aagacacgac | ttatcgccac | tggcagcagc | 4200 |
| 4201 | cactggtaac | aggattagca | gagcgaggta | tgtaggcggt | gctacagagt | 4250 |
| 4251 | tcttgaagtg | gtggcctaac | tacggctaca | ctagaaggac | agtatttggt | 4300 |
| 4301 | atctgcgctc | tgctgaagcc | agttaccttc | ggaaaaagag | ttggtagctc | 4350 |
| 4351 | ttgatccggc | aaacaaacca | ccgctggtag | cggtggtttt | tttgtttgca | 4400 |
| 4401 | agcagcagat | tacgcgcaga | aaaaaaggat | ctcaagaaga | tcctttgatc | 4450 |
| 4451 | ttttctacgg | ggtctgacgc | tcagtggaac | gaaaactcac | gttaagggat | 4500 |
| 4501 | tttggtcatg | agattatcaa | aaaggatctt | cacctagatc | cttttaaatt | 4550 |
| 4551 | aaaaatgaag | ttttaaatca | atctaaagta | tatatgagta | aacttggtct | 4600 |
| 4601 | gacagttacc | aatgcttaat | cagtgaggca | cctatctcag | cgatctgtct | 4650 |
| 4651 | atttcgttca | tccatagttg | cctgactccc | cgtcgtgtag | ataactacga | 4700 |
| 4701 | tacgggaggg | cttaccatct | ggccccagtg | ctgcaatgat | accgcgagac | 4750 |
| 4751 | ccacgctcac | cggctccaga | tttatcagca | ataaaccagc | cagccggaag | 4800 |
| 4801 | ggccgagcgc | agaagtggtc | ctgcaacttt | atccgcctcc | atccagtcta | 4850 |
| 4851 | ttaattgttg | ccgggaagct | agagtaagta | gttcgccagt | taatagtttg | 4900 |
| 4901 | cgcaacgttg | ttgccattgc | tgcaggcatc | gtggtgtcac | gctcgtcgtt | 4950 |
| 4951 | tggtatggct | tcattcagct | ccggttccca | acgatcaagg | cgagttacat | 5000 |
| 5001 | gatcccccat | gttgtgcaaa | aaagcggtta | gctccttcgg | tcctccgatc | 5050 |
| 5051 | gttgtcagaa | gtaagttggc | cgcagtgtta | tcactcatgg | ttatggcagc | 5100 |
| 5101 | actgcataat | tctcttactg | tcatgccatc | cgtaagatgc | ttttctgtga | 5150 |
| 5151 | ctggtgagta | ctcaaccaag | tcattctgag | aatagtgtat | gcggcgaccg | 5200 |
| 5201 | agttgctctt | gcccggcgtc | aacacgggat | aataccgcgc | cacatagcag | 5250 |
| 5251 | aactttaaaa | gtgctcatca | ttggaaaacg | ttcttcgggg | cgaaaactct | 5300 |
| 5301 | caaggatctt | accgctgttg | agatccagtt | cgatgtaacc | cactcgtgca | 5350 |
| 5351 | cccaactgat | cttcagcatc | ttttactttc | accagcgttt | ctgggtgagc | 5400 |
| 5401 | aaaaacagga | aggcaaaatg | ccgcaaaaaa | gggaataagg | gcgacacgga | 5450 |
| 5451 | aatgttgaat | actcatactc | ttcctttttc | aatattattg | aagcatttat | 5500 |
| 5501 | cagggttatt | gtctcatgag | cggatacata | tttgaatgta | tttagaaaaa | 5550 |
| 5551 | taaacaaata | ggggttccgc | gcacatttcc | ccgaaaagtg | ccacctgacg | 5600 |
| 5601 | tctaagaaac | cattattatc | atgacattaa | cctataaaaa | taggcgtatc | 5650 |
| 5651 | acgaggccct | ttcgtcttca | agaa | 5674 |
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pET-11d
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