- 询价
- EASYBIO
- BE3364-100
- 2025年12月21日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Cytochrome C Mouse Monoclonal Antibody
- 规格：
100μl/支
The cytochrome complex, or cyt c is a small hemeprotein found loosely associated with the inner membrane of the mitochondrion. It belongs to the cytochrome c family of proteins
Product Name:Cytochrome C Mouse Monoclonal Antibody
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-5,000 IHC: 1:500-1,000
Optimal dilutions should be determined by the end user.
Specificity:Antibody can detects endogenous Cytochrome C protein.
Alternative Names: CYC antibody, CYCS antibody, HCS antibody, THC4 antibody, Cytochrome C somatic antibody
Form: Liquid
Reactivity:H, R, M
Molecular Weight:14KD
Western blot analysis of 1) Hela, 2) 293T, 3)3T3, 4)Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue, with Cytochrome C Mouse mAb BE3364 diluted at 1:2,000.
Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using Cytochrome C （BE3364）Mouse mAb diluted at 1:500.
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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