Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription
Product Name:Smad3 Rabbit Polyclonal Antibody
Isotype: IgG
Storage Buffer :Antigen Affinity Purified IgG in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-2,000 IHC: 1:200-500
Optimal dilutions should be determined by the end user.
Specificity:Antibody can detects endogenous Smad3 protein.
Alternative Names: hMAD3 antibody, HSPC193 antibody, LDS1C antibody, MADH3 antibody, JV15 2 antibody
Form: Liquid
Reactivity: H
Molecular Weight: ~50KD
Western blot analysis of A431, with Smad3 Rabbit pAb BE3380 diluted at 1:2,000.
Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using Smad3(BE3380)Rabbit pAb diluted at 1:500.