Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.
Product Name:CK7 Mouse Monoclonal Antibody
Clone Number: 2D8
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-2,000 IHC: 1:200 IP: 1:200
Optimal dilutions should be determined by the end user.
Specificity: The CK7 Mouse Monoclonal antibody detects endogenous CK7 proteins.
Molecular Weight:54KD
Form: Liquid
Reactivity:H, R, M
Western blot analysis of 1) Hela, 2)Mouse Kidney, 3) Mouse Brain with CK7 mAb diluted at 1:2,000..
IHC staining of human lung cancer tissue with CK7 mouse mAb(2D8) diluted at 1:200.
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : BE3128 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(BE0105), 1:5,000