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文献和实验dT cellulose into a 2 ml Sarstedt screw cap tubes.2. Make fresh buffers: Note: Amounts are for 2 preps; scale up as necessary.♦ 2X NETS may need to be heated to go into solution.♦ 1X ETS should be heated to 65 ℃ for use.Protocol1. Resuspend
Acetone precipitation of protein
volume of acetone to -20°C. 2. Place protein sample in acetone-compatible tube, such as polypropylene and able to hold six times the sample. Screw cap tubes may help minimize sample losses. 3. Add six times the sample volume of cold (-20°C) acetone
above 18,000 RPM. For work in the higher speed ranges, centrifuge tubes are made of plastic or nitrocellulose. Preparative centrifuge tubes are made of polypropylene (sometimes polyethylene) and can withstand speeds up to 20,000 RPM. These tubes
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