384-well Sample Plates 384孔样品板

Cleanup of the 384-well thermocycler plates for re-use

1.Wash the plates briefly using the 96-channel block washer. 2.Place the plates in a tub submerged in dd-water. 3.Put a flask on top of the plates to keep them submerged. 4.Place the tub with the submerged into the autoclave and sterilize

Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates

and not lose any sample. 4.Rinse the samples by adding 30 μl 70% EtOH to each well of the 384 well plate using the V-prep protocol without mixing. Seal with a Silicone Sealing Mat. Then stack the plates in the swinging bucket of either the Beckman CS-6R

384-well PCR

plates #TF-0384) or 16.3ml per plate, if PCR volume is 40µl/well (maximum for new ABT-384 plates #AB-0937). Add the enzyme last. We were told that Pfu polymerase can destroy primers if PCR mix stored for too long at room temperature