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文献和实验3 times with 500μl wash solution opellet silica once more to remove any residual wash solution oair dry to remove any residual EtOH 3.Elute DNA from silica oresuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer
(10 seconds in 'nanofuge' or brief spin at full spend in microfuge) wash silica 3 times with 500 ul wash solution pellet silica once more to remove any residual wash solution air dry to remove any residual EtOH 3.Elute DNA from silica
Hints and precautions for the care, feeding and breeding of Neurospora
transfer of "stem" stocks, to avoid acquisition of mutations and rearrangements. Subculture from the primary stock to make a working stock for fertilizing crosses, etc. The primary stock should promptly be put into suspended animation, such as silica gel
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